肿瘤药学2018，Vol.8Issue(1)：20-25,6.DOI:10.3969/j.issn.2095-1264.2018.01.05
白藜芦醇通过β-catenin/TCF-4信号通路对胃癌细胞增殖活性及侵袭迁移能力的影响
Effects of Resveratrol on Proliferation Activity and Invasion Ability of Gastric Cancer Cells by β-catenin/TCF-4 Signaling Pathway
摘要
Abstract
Objective To analyze the effect of resveratrol on proliferation activity and invasion ability of gastric cancer cells by regulating β-catenin/TCF-4 signaling pathway.Methods Methylthio tetrazole(MTT)assay was used to explore the appropriate concentration of res-veratrol on the growth and proliferation of gastric cancer cell line MGC-803. Morphological changes of the cells were observed by inverted microscope. The mRNA expression of β-catenin and TCF-4 in Wnt signaling pathway were detected by RT-PCR. The protein expression level of β-catenin and TCF-4 were detected by Western blot. The invasive ability changes of MGC-803 cells were detected by Transwell. Results MTT results showed that resveratrol could significantly inhibit the growth and proliferation of MGC-803 cells. The morphologi-cal changes of MGC-803 cells were observed obviously under inverted microscope. RT-PCR showed that resveratrol could decrease the mRNA expression of β-catenin and TCF-4. The western blot results showed that the protein expressions of β-catenin and TCF-4 were down-regulated by resveratrol. The Transwell results showed that resveratrol significantly decreased the invasive ability of MGC-803 cells. Conclusion Resveratrol can significantly inhibit the proliferation and invasion of gastric cancer cells, and its mechanism may be achieved by inhibiting of β-catenin/TCF-4 signaling pathway.关键词
白藜芦醇/胃癌细胞/β-catenin/TCF-4信号通路Key words
Resveratrol/Gastric cancer cells/β-catenin/TCF-4 signaling pathway分类
医药卫生引用本文复制引用
陈茹,王海燕,苏莹,徐蓉,柳江..白藜芦醇通过β-catenin/TCF-4信号通路对胃癌细胞增殖活性及侵袭迁移能力的影响[J].肿瘤药学,2018,8(1):20-25,6.基金项目
新疆维吾尔自治区人民医院院内项目(20130113) （20130113）
新疆维吾尔自治区自然科学基金(2014211C132). （2014211C132）
