肿瘤药学2018，Vol.8Issue(1)：35-39,5.DOI:10.3969/j.issn.2095-1264.2018.01.08
STIM1基因干扰对人直肠癌SW837细胞生物学功能的影响
The Effects of STIM1 Gene Interference on Biological Functions of Human Rectal Cancer Cells SW837
朱文博 1佘明金 1李桂芝 1胡宏霞 1段婷梅 1程蒙蒙 1简晓红2
作者信息
- 1. 武警安徽省总队医院内三科,安徽 合肥,230061
- 2. 湖南师范大学医学院,湖南 长沙,410013
- 折叠
摘要
Abstract
Objective To study the effect of STIM1 gene interference on cell proliferation, apoptosis and migration ability in vitro in hu-man rectal cancer cells SW837, which could provide a new target for the clinical therapy and drug development of rectal cancer. Methods Lentivirus transfection was used to realize the STIM1 gene interference in human rectal cancer cells SW837. The effects of STIM1 gene interference were verified by quantitivereal-time PCR and western blot. The cell proliferation was analyzed by MTT method, and wound scratch assay was used to detect cell migration ability, the cell apoptosis rate was detected by flow cytometry. The apoptotic proteins expres-sion levels of caspase-3, Bax and Bcl-2 were detected by western blot. Results The levels of STIM1 mRNA and protein in human rectal cancer cells SW837 were significantly decreased after interference lentivirus transfection. After STIM1 interference, the cell proliferation and migratory ability of SW837 cells in vitro decreased (P<0.05). The cell apoptosis rate significantly increased after STIM1 gene interfer-ence (P<0.05), along with the caspase-3 and Bax protein expression levels up-regulation, and the Bcl-2 expression level down-regulation. Conclusion Gene interference of STIM1 in human rectal cancer cells SW837 inhibits the cell proliferation, migration ability in vitro, and enhances the cell apoptosis rate.关键词
人直肠癌细胞/STIM1基因/细胞增殖/凋亡/迁移Key words
Human rectal cancer/STIM1 gene/Cell proliferation/Apoptosis/Migration分类
医药卫生引用本文复制引用
朱文博,佘明金,李桂芝,胡宏霞,段婷梅,程蒙蒙,简晓红..STIM1基因干扰对人直肠癌SW837细胞生物学功能的影响[J].肿瘤药学,2018,8(1):35-39,5.
