中山大学学报（医学科学版）2018，Vol.39Issue(1)：26-33,8.
miR-133b在甲基苯丙胺诱导PC12细胞凋亡中的调控作用
Regulation of miR-133b on Methamphetamine-induced Neuronal Apoptosis in PC12 Cells
摘要
Abstract
[Objective]To investigate the expression changes of miR-133b in methamphetamine(MA)-induced neuro-nal injury in PC12 cells and its regulative effects on cellular apoptosis.[Methods]PC12 cells were cultured and divided into control group and MA treated group.In MA treated group,PC12 cells were insulted with 800μmol/L MA in culture medium. The cellular injury of PC12 cells was observed under microscope. The cellular apoptosis was detected by Hoechst33342/PI double staining,and the expression level of miR-133b was examined by real-time quantitative PCR(RT-PCR). Further-more,miR-133b mimic and inhibitors were transfected into PC12 cells to analyze miR-133b's function in MA-induced cell apoptosis.[Results]The data showed that 800 μmol/L MA could induce obvious cellular injury,cause neurite shortened and increase the cell apoptosis. The RT-PCR data showed that the expression of miR-133b of PC12 cells treated with MA de-creased significantly.The apoptosis rate of PC12 cells decreased after transfection of miR-133b mimic,while increased after transfection of miR-133b inhibitors.[Conclusions]High concentration of MA causes neuron damage and induces neuronal apoptosis,and also decreases the levels of miR-133b expression. Whereas,overexpression of miR-133b can reduce the apoptosis of cultured PC12 cells.Thus,miR-133b plays a crucial role in MA mediated neurotoxicity.This study provides a theoretical basis for elucidating the mechanism of MA-induced neurotoxicity and may provide a new strategy for treating MA addiction.关键词
miR-133b/甲基苯丙胺/神经损伤/凋亡Key words
miR-133b/methamphetamine/neuronal injury/apoptosis分类
医药卫生引用本文复制引用
刘海莉,李涛,王洪杰,胡涛,扈燕来,张静,孙晋浩,董晓光..miR-133b在甲基苯丙胺诱导PC12细胞凋亡中的调控作用[J].中山大学学报（医学科学版）,2018,39(1):26-33,8.基金项目
山东省医药卫生科技发展项目(2013WS0008) （2013WS0008）
山东省重点研发计划(2016GSF201054) （2016GSF201054）
