中山大学学报（医学科学版）2018，Vol.39Issue(1)：34-40,7.
KIF23基因沉默对肝癌HepG2细胞增殖、迁移及侵袭的影响
Effects of KIF23 Gene Silencing on Proliferation,Migration and Invasion of Human Hepatocellular Carcinoma HepG2 cells
摘要
Abstract
[Objective]To investigate the effect of KIF23 gene expression on the proliferation,migration and invasion of human hepatocellular carcinoma HepG2 cells in vitro,and to explore the possible mechanism.[Methods]The KIF23 siRNA was transfected into HepG2 cells by lipofectamine 3000.The expression of KIF23 mRNA and protein in HepG2 cells was de-tected by qRT-PCR and Western blot.The effect of silencing KIF23 on the proliferation of HepG2 cells was studied by CCK-8 assay and plate clone formation assay.The tumor cell abilities of migration and invasion after transfection were measured by scratch assay and Transwell assay.The expression of protein kinase B(PKB/Akt)and phosphorylated Akt(p-Akt)protein in HepG2 cells transfected with KIF23-siRNA2 was detected by Western blot.[Results]KIF23-siRNA could effectively si-lence the expression of KIF23 mRNA and protein in HepG2 cells(P<0.01).The results of CCK-8 assay,plate clone forma-tion assay,scratch assay and Transwell assay demonstrated that the cell proliferation,migration and invasion ability of the KIF23-siRNA2 interference group were significantly inhibited,compared to the negative control group and the blank control group(P<0.05).The expression level of total Akt protein in HepG2 cells was not changed,but the expression level of phos-phorylated Akt protein was down-regulated(P<0.05).[Conclusions]KIF23 may promote the proliferation,migration and in-vasion of human hepatocellular carcinoma cells by activating Akt signal transduction pathway.KIF23 is expected to be a new target for gene therapy of hepatocellular carcinoma.关键词
肝细胞癌/RNA干扰/KIF23/细胞增殖/迁移/侵袭Key words
hepatocellular carcinoma/RNA interference/KIF23/cell proliferation/migration/invasion分类
医药卫生引用本文复制引用
刘素娟,林曲,柏明军,周楚人,陈俊伟,吴春,黄明声..KIF23基因沉默对肝癌HepG2细胞增殖、迁移及侵袭的影响[J].中山大学学报（医学科学版）,2018,39(1):34-40,7.基金项目
广东省自然科学基金面上项目(S2012010008705) （S2012010008705）
广东省自然科学基金博士启动项目(2015A030310171) （2015A030310171）
