中山大学学报（医学科学版）2018，Vol.39Issue(1)：41-47,7.
矢车菊素-3-葡萄糖苷抑制血小板趋化因子介导的THP-1细胞迁移
Cyanidin-3-o-β-glucoside Inhibits THP-1 Cells Migration Mediated by Platelet Derived Chemotactic Factor
摘要
Abstract
[Objective]To evaluate the effects of anthocyanin cyanidin-3-o-β-glucoside(Cy-3-g)on monocyte migra-tion mediated by platelet derived factors in vitro.[Methods]Thrombin-activated human gel-filtered platelets were incubated with different concentrations(0,0.5,5 or 50 μmol/L)of Cy-3-g.The level of TGF-β1,β-TG,CCL5 in platelet superna-tant was determined by ELISA.The peripheral venous blood from healthy volunteers were incubated with different concentra-tions of Cy-3-g. The expression of CCR5 on leukocytes was detected by flow cytometer. Calcein AM labeled THP-1 cells were incubated with the releasates of Cy-3-g-treated gel-filtered platelets for 120 min,and then the number of the THP-1 cells were counted by a microscope.Moreover,THP-1 cells were pre-incubated with different concentrations of Cy-3-g with or without CCR5 inhibitor maraviroc(200 μmol/L)for 60 min,then treated with recombinant CCL5(100 nmol/L)for 120 min.The number of the THP-1 cells were counted as well.[Results]Cy-3-g significantly inhibited platelet TGF-β1,β-TG,CCL5 secretion and decreased CCR5 expression of leukocytes compared with the control(P<0.05).Moreover,the sig-nificant inhibitory effects of Cy-3-g on THP-1 cell migration toward the releasates of Cy-3-g-treated platelets were also ob-served(P<0.05). In addition,THP-1 cell migration toward recombinant CCL5 was significantly suppressed by Cy-3-g.[Conclusions]Anthocyanin Cy-3-g inhibited inflammation of atherosclerosis by platelet TGF-β1,β-TG,CCL5 secretion and CCL5-mediated monocyte migration.Our results provided new ideas for prevention and treatment of atherosclerosis.关键词
花色苷/血小板趋化因子/THP-1细胞/CCL5/CCR5Key words
anthocyanin/platelet chemokines/THP-1/CCL5/CCR5分类
医药卫生引用本文复制引用
陈彦球,张献丹,覃楠,李卿,杨燕..矢车菊素-3-葡萄糖苷抑制血小板趋化因子介导的THP-1细胞迁移[J].中山大学学报（医学科学版）,2018,39(1):41-47,7.基金项目
国家自然科学基金(81573145,No.81372978) （81573145,No.81372978）
广东省科技计划项目(2015A050502020,2015A030313117) （2015A050502020,2015A030313117）
