中山大学学报（医学科学版）2018，Vol.39Issue(1)：87-92,6.
不同时相慢加急性乙肝肝衰竭患者外周血NK细胞及其受体分析
Analysis of NK Cells and Receptors in Peripheral Blood of Patients with HBV Related Acute-on-chronic Liver Failure in Different Phases
摘要
Abstract
[Objective]To investigate the character of expression of NK cells and its receptors in peripheral blood of pa-tients with HBV-related acute-on-chronic liver failure(HBV-ACLF)in different phase.[Methods]There are thirteen pa-tients with HBV-ACLF in advanced phase group and thirty-three patients in plateau phase group,with thirteen healthy per-son in control group.The frequency of NK cells and expression of NK cell activating receptors(NKAR)including NKG2D, NKp30 and NKp46,NK cell inhibitory receptors(NKIR))cludingsd ate the character of including NKG2A,KIR2DL1, KIR2DL3 and KIR3DL1,NK cell killing function related factors including Perforin,GranzymeB and FasL in peripheral blood of all patients were detected by flow cytometry.[Results]The percentage of NK cells in advanced group was lower than plateau group and healthy control group(H=7.771,P=0.021).The expression of KIR3DL1 in healthy control group was high-er than advanced group and plateau group(Z=6.639,P=0.036)while the expression of FasL was significantly lower than ad-vanced group and plateau group(Z=22.5,P<0.001).[Conclusion]Patients in advanced group had lower frequency of NK cells,lower expression of inhibitory receptor KIR3DL1 and higher expression of FasL than patients in plateau group and healthy control group,which is associated with immune status of patients in different phase of liver failure.关键词
乙型肝炎/慢加急性肝衰竭/NK细胞/免疫细胞受体Key words
Hepatitis B virus/Acute-on-chronic liver failure/Natural killer cell/Receptors of immunocytes分类
医药卫生引用本文复制引用
翁伟镇,熊静,曹会娟,陈俊峰,张绍全,张静,林炳亮..不同时相慢加急性乙肝肝衰竭患者外周血NK细胞及其受体分析[J].中山大学学报（医学科学版）,2018,39(1):87-92,6.基金项目
"十二五"国家科技重大专项(2012ZX10002004) （2012ZX10002004）
广州市产学研协同创新重大专项(1561000157) （1561000157）
