中山大学学报（医学科学版）2018，Vol.39Issue(1)：93-100,8.
精子DNA碎片率及顶体酶活性对男性不育的诊断价值
Diagnostic Value of DNA Fragmentation Index and Acrosin Activity in Male Infertility
摘要
Abstract
[Objective]We aimed to explore the diagnostic value of DNA fragmentation index(DFI)and acrosin activi-ty in male infertility.[Methods]Nine hundred and two semen samples were collected from patients and assessed for the DNA fragmentation index by sperm chromatin dispersion test,acrosin activity,as well as standard sperm parameters according to the WHO criteria. Statistical analysis was performed with SPSS.[Results]Statistically significant differences were observed in age,sperm concentration,total motility,progressive rate and acrosin activity among different group of sperm DNA dam-age(≤10%,11~20%,21~30%,≥31%).Sperm acrosin activity also showed difference in sperm concentration,total num-ber,total motility,progressive rate,normal morphology rate,teratozoospermia index(TZI)and sperm DFI.The DNA frag-mentation rate and sperm concentration,sperm motility,forward motility rate,total sperm count and acrosin activity was neg-atively correlated,while it is negative correlatd with TZI. Acrosin activity and sperm concentration,sperm motility,forward motility rate,sperm count and normal morphology rate was positively correlated,while it is negative correlated with the ab-normal rate of head,sperm deformity index,DNA fragmentation rate.[Conclusion]Sperm DNA damage and acrosin activity could partly reflect the quality of sperm. Moreover,sperm DFI maypredict the sperm motility part while the acrosin activity more likely related to sperm morphology.关键词
男性不育/精子/DNA碎片率/顶体酶活性Key words
male infertility/spermatozoa/DNA fragmentation index/acrosine activity分类
医药卫生引用本文复制引用
张宁锋,刘彩霞,郑灵燕,区颂邦,梁海霞,钟慧玲,陈静华..精子DNA碎片率及顶体酶活性对男性不育的诊断价值[J].中山大学学报（医学科学版）,2018,39(1):93-100,8.基金项目
广东省自然科学基金(2016A030313071) （2016A030313071）
