组织工程与重建外科杂志2018，Vol.14Issue(1)：24-27,4.DOI:10.3969/j.issn.1673-0364.2018.01.007
骨髓间充质干细胞对七氟烷所致的原代神经元凋亡及细胞活性的影响
Effects of Bone Marrow Mesenchymal Stem Cells on Sevoflurane-Induced Apoptosis and Cell Viability of Primary Cultured Neuron
程燕咏 1王佳怡 1蒋云凤 1孙宇1
作者信息
- 1. 200011上海市 上海交通大学医学院附属第九人民医院麻醉科
- 折叠
摘要
Abstract
Objective To explore the effects of bone marrow mesenchymal stem cells(BMSCs)on apoptosis,cell viability and the level of TNFα of primary cultured neuron. Methods BMSCs and primary cultured neuron were cultured in non-contact co-culture system by Transwell plate.After the exposure to 4% sevoflurane for 6 hours,the morphological changes of primary cultured neuron were observed. Western Blot was used to analyze cell apoptosis; MTT and LDH were used to detect cell viability;ELISA was used to determine the level of TNFα.Results The cell morphological changes, cell apoptosis, cell viability decline and TNFα release of primary cultured neuron induced by sevoflurane were all decreased after BMSCs preprocessing(P<0.05).Conclusion BMSCs preprocessing can suppress the neurotoxic injury induced by sevoflurane.关键词
七氟烷/间充质干细胞/原代神经元/细胞凋亡/细胞活性Key words
Sevoflurane/Mesenchymal stem cells/Primary cultured neuron/Apoptosis/Cell viability分类
医药卫生引用本文复制引用
程燕咏,王佳怡,蒋云凤,孙宇..骨髓间充质干细胞对七氟烷所致的原代神经元凋亡及细胞活性的影响[J].组织工程与重建外科杂志,2018,14(1):24-27,4.
