重庆医学2018，Vol.47Issue(5)：607-609,613,4.DOI:10.3969/j.issn.1671-8348.2018.05.010
抗坏血酸对钴纳米粒子及钴离子致成纤维细胞毒性的保护作用
Protective effect of L-ascorbic acid against fibroblast cytotoxicity induced by cobalt nanoparticles and ions
摘要
Abstract
Objective To explore the effect of L-ascorbic acid(AA) for reducing the fibroblast cells induced by cobalt(co)cytotoxicity.Methods The experiment was divided into the blank control group,Co2+ group,Co2++AA group,cobalt nanoparticles(CoNPs) group,CoNPs+AA group and AA group.The AA pretreated for 1 h was performed in advance.The CCK8 assay was used to detect the CoNPs and Co2+ induced and AA-treated cytotoxicity.The fluorescence staining was used to measure the production of ROS in mitochondria.The real-time polymerase chain reaction(PCR) and Western blot were used to measure the expression of related protein and related molecular mRNA respectively.Moreover the cytochrome-C level in cytoplasml was detected.Results After treating the cells by CoNPs and Co2+,the cells appeared apoptosis.CoNPs could significantly induce ROS generation;the proapoptotic factors(caspases-3,and Bax) were significantly increased,while the anti-apoptotic factor Bcl-2 expression was decreased;cytochrome C and AIF expressions were up-regulated and released from mitochondria into the cytoplasm,after AA pretreatment,these changes caused by CoNPs were decreased.Conclusion AA can reduce the CoNPs caused cytotoxicity by reducing ROS generation and release,but can not weaken the Co2+ toxic effect.关键词
钴/纳米粒子/氧化性应激/细胞凋亡/抗坏血酸Key words
cobalt/nanoparticles/oxidative stress/apoptosis/ascorbic acid分类
医药卫生引用本文复制引用
洪鸿翔,朱海,刘雅克,杨晓佑,吴雪飞,崔志明,刘璠..抗坏血酸对钴纳米粒子及钴离子致成纤维细胞毒性的保护作用[J].重庆医学,2018,47(5):607-609,613,4.基金项目
南通市市级科技计划项目(MS12017001-7). （MS12017001-7）
