重庆医学2018，Vol.47Issue(5)：610-613,4.DOI:10.3969/j.issn.1671-8348.2018.05.011
慢性缺血性脑白质脱髓鞘比格犬模型中Tau蛋白表达及可能原因
Tau protein expression and possible reasons in Beagle dog model of chronic ischemic cerebral white matter dysmyelination
章洪智 1刘静 2杨树 2王多姿 2魏本 3郭富强2
作者信息
- 1. 电子科技大学医学院生物医学工程,成都 610054
- 2. 四川省医学科学院/四川省人民医院/电子科技大学附属医院神经内科,成都 610072
- 3. 中国人民解放军成都军区总医院神经内科,成都 610083
- 折叠
摘要
Abstract
Objective To explore the expression level and possible reasons of Tau protein in Beagle dog model of ischemic cerebral white matter(WM) demylination.Methods Sixteen adult Beagle dogs were divided into the sham operation group(A) and observation group B,C and D according to the completely random mumber table method,4 cases in each group.Different.degrees of cerebral ischemia animal models were established by 4-vessel occlusion(4-VO) method.The bilateral ventricle edge white matter (WM) was selected.The oligodendrocyte precursors(OPCs) were labeled by NG2.The mature oligodendrocytes(Ols) were labeled by CNPase.Tau,NG2 and CNPase were detected by using the immunohistochemical method.The expression level was quantified by the mean optical value.The correlation among Tau,NG2 and CNGase was analyzed by adopting the Pearson linear correlation analysis.Results The HE staining showed obvious changes of WM demylination after chronic cerebral ischemic.The scores after LFB staining in the group A,B,C and D were(0.75 ± 0.71) points,(1.38 ± 0.06) points,(1.63 ± 0.52) points and (1.88 ± 0.64)points.Compared with the group A,the scores in the group B,C and D were much higher(P＜0.05).Compared with the group A,the expression levels of Tau protein and NG2 were significantly increased(P＜0.05),while the CNPase expression level was significantly decreased(P＜0.05).The Pearson correlation analysis showed that Tau expression level was positively correlated with the NG2 expression level(r=0.277,P=0.006);Tau and NG2 were negatively correlated with the CNPase level(r=-0.303,-0.402,P=0.003,0.001).Conclusion The increase of Tau expression in Beagle dog model of ischemic cerebral WM demylination may be related to the differentiation dysmaturity of OPCs.关键词
Tau蛋白/脑白质脱髓鞘/慢性脑缺血/少突胶质前体细胞/少突神经胶质Key words
Tau protein/cerebral white matter demylination/chronic cerebral ischemic/oligodendrocyte precursors cells/oligodendroglia分类
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章洪智,刘静,杨树,王多姿,魏本,郭富强..慢性缺血性脑白质脱髓鞘比格犬模型中Tau蛋白表达及可能原因[J].重庆医学,2018,47(5):610-613,4.
