重庆医学2018，Vol.47Issue(5)：622-625,4.DOI:10.3969/j.issn.1671-8348.2018.05.014
类风湿关节炎患者外周血Treg细胞及其分泌IL-35水平检测与临床意义
Detection of peripheral blood Treg cells and secreted IL-35 level in patients with rheumatoid arthritis and its clinical significance
摘要
Abstract
Objective To detect the number of peripheral blood Treg cells and secreted IL-35 expression level in the patients with rheumatoid arthritis(RA) and to explore their correlation with RA occurrence.Methods Peripheral blood was collected from 45 cases of RA,22 cases of osteoarthritis(OA) and 26 persons undergoing healthy physical examination(control group).The number of CD4+ CD25+ foxp3+ regulatory T cells was determined by flow cytometry,while plasma IL-35 level was determined by ELISA.Then the relationship between Treg and expressed IL-35 with clinical indicators was analyzed.Results The percentage of peripheral blood Treg ceils to total CD4+T cells in the RA group was (5.65 ± 2.33)％,which was significantly increased compared with (4.12 ± 1.75) ％ in the control group(P＜0.05);the difference between the OA group and RA group was not statistically significant(P=0.086).The average fluorescence intensity of foxp3 had no statistical difference among 3 groups(P＞0.05).The higher the DAS28 score,the lower the peripheral blood Treg cells number and foxp3 fluorescence intensity.The plasma IL-35 level in the RA group [(34.22± 14.35)ng/L] was significantly lower than that in the OA group[(78.63± 24.58)ng/L] and control group [(67.56±25.43)ng/L],the difference was statistically significant(P＜0.05).The Treg number in RA patients was negatively correlated with ESR and DAS28 score(r=-0.223,-0.343,P=0.023,0.011),but had no correlation with rheumatoid factor,C-reactive protein and anti-CCP antibody.Conclusion The peripheral blood Treg cells number in RA patients is elevated,while the IL-35 level is decreased,the negative regulation ability in the patients with Treg cell function deficit is attenuated.关键词
类风湿关节炎/骨关节炎/Treg细胞/白细胞介素35/流式细胞术Key words
rheumatoid arthritis/osteoarthritis/Treg cells/interleukin-35/flow cytometry分类
医药卫生引用本文复制引用
熊怡淞,宋燕,俞娟,陈莉..类风湿关节炎患者外周血Treg细胞及其分泌IL-35水平检测与临床意义[J].重庆医学,2018,47(5):622-625,4.基金项目
国家自然科学基金资助项目(81302596、81301484) （81302596、81301484）
成都军区总医院院管课题(2013YG-B064). （2013YG-B064）
