重庆医学2018，Vol.47Issue(5)：654-656,659,4.DOI:10.3969/j.issn.1671-8348.2018.05.024
UAS-Hey转基因果蝇品系的构建与鉴定
Construction and identification of UAS-Hey transgenic fly strains
摘要
Abstract
Objective To construct the UAS-Hey transgenic fly strains to provide a tool for researching the function of Hey gene in the fly development.Methods The Hey gene coding sequence was amplified by reverse transcription PCR and subcloned into pUAST expression vector.The pUAS-Hey recombinant plasmid was constructed and microinjected into the embryos of wild type flies.The UAS-Hey transgenic flies with red eyes were screened out with mini-white marker,then the balancing and mapping of these transgenic strains were performed.The identification was performed by using the PCR amplification method.Results The pUAS-Hey recombinant plasmid was successfully constructed,and seven independent transgenic strains were obtained by microinjection and transgenic fly screening and balance.PCR analysis confirmed that the P[mini-white,UAS-Hey] was integrated into genomes of transgenic strains and was in expressible region.Conclusion The UAS-Hey transgenic flies are successfully constructed,which lays the foundation for further studies of the function and regulation of Hey gene with GAL4/UAS system.关键词
Hey基因/转基因果蝇/显微注射/心血管发育Key words
Hey gene/transgenic fly/microinjection/cardiovascular development分类
医药卫生引用本文复制引用
刘松年,荆凌华,伍星,赵鑫..UAS-Hey转基因果蝇品系的构建与鉴定[J].重庆医学,2018,47(5):654-656,659,4.基金项目
洛阳市科技计划项目(1503007A-2). （1503007A-2）
