重庆医学2018，Vol.47Issue(6)：728-732,5.DOI:10.3969/j.issn.1671-8348.2018.06.003
溪黄草对肝癌HepG2细胞基因表达谱的影响
Effect of rabdosia serra (maxim.) hara on gene expression profile of hepatocellular carcinoma HepG2 cells
摘要
Abstract
Objective To adopt the expression profile chip to investigate the effect of Rabdosia serra (Maxim.) hara water extract on related gene of human hepatocellular carcinoma(HCC) HepG2 cells for researching the possible mechanism of its water extract on HCC.Methods The human HCC HepG2 cells were cultured in vitro.After adding Rabdosia serra (Maxim.) hara water extract (9.54 mg/mL) for 24 h action,the expression profile chip was adopted to detect the HepG2 gene change after Rabdosia serra (Maxim.) hara action and then the chip results were verified by using RT-PCR and Western blot.Results The phase contrast microscope observation found that compared with the negative control group,the number of HepG2 cells was significantly reduced after Rabdosia serra (Maxim.) hara water extract action.The expression profile results showed that after Rabdosia serra (Maxim.)hara water extract action for 24 h,264 genes were risen to over twice folds compared with the negative control group and 194 genes were decreased by over twice folds compared with the negative control group.The gene ontology(GO) and KEGG analysis indicated that Rabdosia serra (Maxim.) hara could up-regulate multiple genes in DUSPs and IGFBPs family and down-regulate multiple genes in MCMs family.The RT-PCR detection found that compared with the negative control group,DUSP1 and IGFBP1 in the Rabdosia serra (Maxim.) hara treatment group were increased,while FXR and ALDH8A1 were decreased (P＜0.01),which were consistent with the results of expression profile chip.The Western blot results found that the protein expression level of DUSP1 in the Rabdosia serra (Maxim.) hara treatment group was also significantly higher than that in the negative control group (P＜ 0.01).Conclusion The expression profile chip shows that Rabdosia serra (Maxim.) hara can inhibit the proliferation of HCC cells by regulating multiple genes.关键词
溪黄草/癌,肝细胞/HepG2/基因表达谱Key words
rabdosia serra hara/carcinoma,hepatocellular/HepG2 cells/gene expression profile分类
医药卫生引用本文复制引用
罗莹,廖长秀,贺珊,赖术,黎为能..溪黄草对肝癌HepG2细胞基因表达谱的影响[J].重庆医学,2018,47(6):728-732,5.基金项目
广西壮族自治区自然科学基金资助项目(2014GXNSFAA118252) （2014GXNSFAA118252）
广西高校科学技术研究项目(KY2015ZD093). （KY2015ZD093）
