重庆医学2018，Vol.47Issue(6)：779-782,785,5.DOI:10.3969/j.issn.1671-8348.2018.06.018
乳腺癌患者外周血miRNA-134表达及化疗耐药的机制
Expression of peripheral blood miRNA-134 in patients with breast cancer and mechanism of chemotherapy drug resistance
顾峰 1周岩 1韦培培1
作者信息
- 1. 上海市浦东新区人民医院检验科 201200
- 折叠
摘要
Abstract
Objective To explore the expression of peripheral blood miRNA-134 in the patients with breast cancer and chemotherapy drug resistance mechanism.Methods A total of 98 cases of newly diagnosed breast cancer were divided into the chemotherapy sensitive group(n=68) and chemotherapy drug resistant group(n=30).The expression of miRNA-134 in peripheral blood was measured by adopting RT-PCR.miRNA-mimics and miRNA-inhibitor were transfected into epirubicin and docetaxel resistant cell lines,the cell apoptosis and expression levels of Bax and Caspase-3 protein were detected.Results The level of serum miRNA134 expression in the chemotherapy sensitive group was significantly higher than that in the chemotherapy drug resistant group (P＜0.05);the proliferation capacity of epirubicin and docetaxel resistant cell lines was significantly lower than that in the MCF-7 cells(P＜0.05).Compared with normal MCF-7 cell line,the expression level of miRNA-134 in the epirubicin and docetaxel resistant cell lines was significantly decreased(P＜0.05).The apoptosis rate in the miRNA-mimics group was significantly higher than that in the control group and miRNA-inhibitor group(P＜0.05).The expression of Bax and Caspase-3 protein in the miRNA-mimics group was significantly higher than that in the control group and miRNA-inhibitor group(P＜0.05).Conclusion MiRNA-134 may enhance the sensitivity of breast cancer ceils to chemotherapy by promoting the expression of Bax and Caspase-3.关键词
乳腺肿瘤/新辅助化疗/耐药/微RNA-134/作用机制Key words
breast neoplasms/neoadjuvant chemotherapy/drug resistance/micro RNA-134/mechanism分类
医药卫生引用本文复制引用
顾峰,周岩,韦培培..乳腺癌患者外周血miRNA-134表达及化疗耐药的机制[J].重庆医学,2018,47(6):779-782,785,5.
