解剖学杂志2018，Vol.41Issue(1)：28-33,6.DOI:10.3969/j.issn.1001-1633.2018.01.008
大鼠中脑水管周围灰质各分区向下丘脑orexin阳性神经元分布区的投射及其联系
Connections between projection fibers from different subdivisions of the periaqueductal gray and orexin-containing neurons of the hypothalamus in rats
摘要
Abstract
Objective:To investigate the organization of projections from different subdivisions of the periaqueductal gray (PAG) to orexin-containing neurons in the hypothalamus and to reveal the morphological basis for the modulatory effects of the PAG innervations on orexin-containing neurons in rats.Methods:Cholera toxin B subunit (CTb) injections were made into the orexin-containing neurons field in the hypothalamus of rats and the distributions of CTb-immunoreactive (ir) neurons in the PAG were observed under a light microscope.An combination of biotinylated dextran amine (BDA) injection into different subdivisions of PAG and immunostaining for orexin-containing neurons was implemented,the distribution of BDA-labeled fibers and the orexin-ir neurons was observed in the hypothalamus.Results:After an unilateral CTb injection into suprafornical area or lateral hypothlamus,numerous CTb-ir neurons were observed bilaterally with a ipsilateral predominance in the PAG.The lateral PAG (lPAG) and ventrolateral PAG (vlPAG) contained a large number of CTb-ir neurons,while dorsolateral PAG (dlPAG) and dorsomedial PAG (dmPAG) contained a few labeled neurons.A small number of CTb-ir neurons were seen in the contralateral portions of PAG.After BDA injection into dlPAG,lPAG and vlPAG respectively and immunostaining of orexin-containing neurons,BDA-labeled fibers were observed bilaterally with a clear-cut ipsilateral predominance in the hypothalamus.The prominent overlap of the distribution field of the BDA-labeled fibers and that of the orexin-ir neurons were found in the suprafornical area.In this area,bouton-like varicosities labeled with BDA from lPAG and vlPAG were often closely apposed to cell bodies and dendrites of the orexin-ir neurons.Very sparse apposition of those BDA labeled fibers from dlPAG and orexin-ir neurons were found.Conclusion:Fibers from lPAG and vlPAG and orexin-ir neurons in the suprafornical area of the hypothalamus are overlapping and are closely connected,which may directly affect the functions of orexin-containing neurons.关键词
中脑水管周围灰质/下丘脑/神经元/orexin/大鼠Key words
periaqueductal gray/hypothalamus/neuron/orexin/rat引用本文复制引用
王晓,张博闻,和祯泉,赵奇,顾金海,何仲义,牛建国..大鼠中脑水管周围灰质各分区向下丘脑orexin阳性神经元分布区的投射及其联系[J].解剖学杂志,2018,41(1):28-33,6.基金项目
国家自然科学基金(81460207) （81460207）
2013年度中国科学院“西部之光”人才培养计划 （）
宁夏卫生计生委重点科研课题(2015-NW-036) （2015-NW-036）
宁夏自治区“十三五”重大科技项目(2016BZ07) （2016BZ07）
