解剖学杂志2018，Vol.41Issue(1)：40-44,5.DOI:10.3969/j.issn.1001-1633.2018.01.011
不同强度运动预处理对全脑缺血再灌注大鼠生长相关蛋白-43、轴突生长抑制因子Nogo-A表达的影响
Effect of different exercise intensity preconditioning on expression of growth associated protein-43 and Nogo-A in rats with cerebral ischemia/reperfusion
摘要
Abstract
Objective:To observe the effect of different exercise intensity preconditioning on expression of growth associated protein-43 (GAP-43) and neurite outgrowth inhibitor-A (Nogo-A) in rats with cerebral ischemia/reperfusion (I/R).Methods:Global cerebral ischemia model was formed by improved Pulsinelli four-vessel occlusion.Totally 80 rats were divided into four groups randomly:sham group,I/R group,exercise intensity 1 preconditioning group and exercise internsity 2 preconditioning group.Rats were sacrificed at the time of 6 h,1 d,3 d and 7 d respectively after injury.Morphological changes of neural cells in hippocarnpal CA1 area were observed by hematoxylin-eosin staining.Expression of GAP-43 and Nogo-A were detected respectively by immunohistochemistry and RT-PCR.Results:Compared with those in the cerebral ischemia/reperfusion group,the number of necrosis neurons significantly decreased and the expression of GAP-43 significantly increased at different time points in exercise intensity 1 preconditioning group and decreased in exercise intensity 2 preconditioning group.Meanwhile,the expression of Nogo-A was significantly decreased in exercise intensity 1 preconditioning group and increased in exercise intensity 2 preconditioning group.Conclusion:Exercise intensity 1 preconditioning can aggravate the apoptosis of nerve cells after cerebral ischemia/reperfusion injury and exercise intensity 2 preconditioning can aggravate the damage and loss of nerve cells,which is related to the regulation of GAP-43 and Nogo-A.关键词
运动预处理/脑缺血再灌注/生长相关蛋白-43/轴突生长抑制因子-A/大鼠Key words
exercise preconditioning/cerebral ischemia/reperfusion/growth associated protein-43/neurite outgrowth inhibitor-A/rat引用本文复制引用
孙竹梅,赵雅宁,李建民,王国立,陈长香,张美航,王静..不同强度运动预处理对全脑缺血再灌注大鼠生长相关蛋白-43、轴突生长抑制因子Nogo-A表达的影响[J].解剖学杂志,2018,41(1):40-44,5.基金项目
河北省卫生厅课题(ZD2010106) （ZD2010106）
唐山市科技计划项目(14130220B) （14130220B）
