水产学报2018，Vol.42Issue(3)：314-321,8.DOI:10.11964/jfc.20161010566
基于3种线粒体基因的珠母贝属系统进化关系
Phylogenetic relationships in Pinctada from Leizhou Peninsula based on 3 mtDNA genes
摘要
Abstract
In oder to reconstruct the molecular phylogeny of Pinctada,partial sequences of 3 mtDNA fragments (12S rRNA,16S rRNA,COⅠ)from 5 species of Pinctada(P.martensii,P.maxima,P.margaritifera,P.maculata, P.nigra)were amplified by polymerase chain reaction(PCR)and directly sequenced,and the samples were collec-ted from Leizhou Peninsula. Base ratio and genetic distances were analyzed. At the same time, these sequences were compared with the published sequence of P.albina.Results showed that A+T contents of 3 mtDNA frag-ments from 5 species were higher than G+C obviously, in accordance with the other invertebrate mtDNA se-quences. Data showed that the sequences of 3 mtDNA fragments had a high degree of saturation. Analysis showed that the molecular phylogenetic trees were basically constructed and well supported the traditional morphological taxonomy.In the relations of P.nigra and P.albina,3 mtDNA fragments were very similar in nucleotide composi-tion,genetic variability,genetic distance and molecular phylogenetic trees.So this suggests that P.nigra and P.al-bina represented two subspecies in the same species.About the taxonomy of P.maculata,the molecular phylogen-etic trees showed that P.maculata was close to P.nigra and P.albina.Moreover,it revealed P.margaritifera and P.maxima were more primitive than the others.There was a little difference on the genetic relationship analysis about P.martensii by 3 mtDNA fragments,and no conclusion could be drawn from the existing data.It is neces-sary to do more study of molecular systems and morphological and anatomical structure.关键词
珠母贝属/线粒体基因/12SrRNA/16SrRNA/COⅠ/分子系统学Key words
Pinctada/mtDNA/12S RNA/16S RNA/COⅠ/molecular phylogenetics分类
生物科学引用本文复制引用
肖宝华,廖宝林,刘楚吾,杨小东,谢子强,张武财..基于3种线粒体基因的珠母贝属系统进化关系[J].水产学报,2018,42(3):314-321,8.基金项目
国家科技支撑计划(2007BAD29B00)National Key Technology R&D Program of the Ministry of Science and Technology of China(2007BAD29B00) （2007BAD29B00）
