水产学报2018，Vol.42Issue(3)：322-333,12.DOI:10.11964/jfc.20161110620
转录组测序研究三角帆蚌珍珠颜色相关基因
Transcriptome sequencing analysis on pearl color related genes in Hyriopsis cumingii
摘要
Abstract
The transcriptome of Hyriopsis cumingii ventral mantle tissue whose nacre colors were purple and white was sequenced by a new generation of high throughput sequencing technology in order to find candidate genes in-volved in pearl color produced by H.cumingii.After quality control and assembly,the sequences acquired were blasted against NR, Swiss-Prot, COG, KOG, KEGG, GO and Pfam databases, and then cluster analysis were per-formed. In total, 72 800 581 bp of clean data, and 89 529 Unigenes were obtained, and 2644 genes were differen-tially expressed, among which 1323 were up-regulated and 1321 down-regulated. The Unigenes GO function in the transcriptome library was divided into 3 categories: molecular function, cellular component and biological pro-cess with 50 branches; it could be divided into 187 classes taking the KEGG database as a reference. Differentially expressed gene analysis revealed that some genes were related to pigment biosynthesis (such as xanthommatin, melanin and pteridine) and metal ion transportation. And there were 6 cytochrome P450, 3 cytochrome b561 and 1 cytochrome b560 genes.They might play important roles in the formation of pearl color of H.cumingii.关键词
三角帆蚌/珍珠颜色/差异表达/转录组测序Key words
Hyriopsis cumingii/pearl color/differential expression/transcriptome sequencing分类
生物科学引用本文复制引用
毛媛媛,郑荣泉,张启鹏,梅祎芸,孔绅绅,刘志芳..转录组测序研究三角帆蚌珍珠颜色相关基因[J].水产学报,2018,42(3):322-333,12.基金项目
浙江省十二五水产育种专项(2012C12907-9 （）
2012C12907-5)Zhejiang Province Twelfth Five-year Special Aquatic Products Breeding(2012C12907-9 （）
2012C12907-5) （）
