水产学报2018，Vol.42Issue(3)：334-344,11.DOI:10.11964/jfc.20170110685
两种方法分析大鳞鲃染色体核型的比较研究
Comparative study on the karyotype of chromosome of Barbus capito with two methods
摘要
Abstract
Chromosome karyotyping is the main content in cytogenetic study. Manual analysis, image systemic analysis and software analysis are the most commonly used methods in chromosome karyotyping. But all three methods have their own disadvantage, which are large measurement error, high quality metaphase image require-ment and uncontrollable detail error, respectively. In order to obtain accurate and convenient chromosome karyo-type analysis method,the introduced salt-tolerant varieties,Barbus capito,was used as experimental fish.The metaphase chromosomes of B.capito were obtained from head kidney tissue by the method of PHA and col-chicine injection and then the chromosome samples were prepared by air-drying technique. We presented a meth-od for chromosome karyotyping by combining two software E-ruler and Photoshop, and compared the measure and karyotyping results from the regular method. The results showed that there is no significant difference in the measurement of stretched straight chromosomes by E-ruler software and conventional methods, however, the former is more accurate in the measurement of curved chromosome. Photoshop software can quickly and conveni-ently complete the chromosome, because it can accurately judge the location of centromere by eliminating the background of karyotype. It can be concluded that E-ruler and Photoshop combining method achieved much more accurate and clear karyotyping results. As an accurate and convenient method, this new method can also be used in chromosome analysis of other fish species and even other organisms. The results showed that the chromosome ploidy of B.capito was diploid,and no gender-related heterotypical chromosomes were found.The karyotype for-mula was:2n=100=12m+38sm+38st+12t,NF=150.Compared with other species from Barbinae family,B.capito has similar karyotypes to several domestic species, which shows the karyotype evolutionary characters.关键词
大鳞鲃/染色体核型/分析方法/细胞遗传学Key words
Barbus capito/chromosome karyotype/analysis method/cytogenetics分类
农业科技引用本文复制引用
耿龙武,姜海峰,徐伟..两种方法分析大鳞鲃染色体核型的比较研究[J].水产学报,2018,42(3):334-344,11.基金项目
中国水产科学研究院基本科研业务费项目(2016HY-ZD0602) （2016HY-ZD0602）
中央级公益性科研院所基本科研业务费项目(HSY201403) （HSY201403）
"十二五"国家科技支撑计划(2012BAD25B09)Central-level Non-profit Scientific Research Institutes Special Funds(2016HY-ZD0602 （2012BAD25B09）
HSY201403) （）
National Key Technology R&D Program of the Ministry of Science and Technology of China(2012BAD25B09) （2012BAD25B09）
