水产学报2018，Vol.42Issue(3)：366-375,10.DOI:10.11964/jfc.20161010584
饲料磷脂对已交配和未交配雌性三疣梭子蟹卵巢发育、组织学和卵黄蛋白原表达的影响
Effects of dietary soy lecithin on the ovary development, histology and vitellogenin gene expression in mated and unmated female swimming crab(Portunus trituberculatus)
摘要
Abstract
A 12-week experiment was conducted to evaluate the effects of dietary soy lecithin on ovary develop-ment, gonadal histology and vitellogenin mRNA expression of swimming crab. 2×2 double-factor random block design was used to arrange 0% or 4% soy lecithin for mated or unmated female swimming crab, respectively. The results indicated that crabs fed the diets containing 4% soy lecithin had significantly higher gonadosomatic index (GSI), oocyte diameter, the levels of serum vitellogenin and sex steroids than those fed the unsupplemented with soy lecithin diets. Hepatopancreatic vitellogenin mRNA expression increasesed with dietary soy lecithin increas-ing from 0% to 4%. The results of hepatosomatic index (HSI) showed a reverse trend with GSI. Mated female crabs exhibited higher hepatopancreatic vitellogenin mRNA expression than unmated female crabs. Furthermore, the mated female crabs had higher ovarian yolk granules in number and size than those unmated female crabs. Mating treatment did not affect HSI, serum vitellogenin (VG) and progesterone (PG) contents. The interaction of dietary soy lecithin and mating on serum progesterone (PG) and estradiol (E2) contents was identified. In conclu-sion, the dietary soy lecithin and mating stimulated had a positive effect on ovarian development in swimming crab broodstock, and mating stimulated had a more beneficial effect on promoting oocyte maturation.关键词
三疣梭子蟹/大豆磷脂/卵巢发育/组织学/卵黄蛋白原Key words
Portunus trituberculatus/soy lecithin/ovary development/gonadal histology/vitellogenin分类
农业科技引用本文复制引用
丁立云,付辉云,侯迎梅,霍雅雯,孙蓬,金敏,周歧存..饲料磷脂对已交配和未交配雌性三疣梭子蟹卵巢发育、组织学和卵黄蛋白原表达的影响[J].水产学报,2018,42(3):366-375,10.基金项目
国家自然科学基金(41476125) （41476125）
浙江省重点研发计划项目(2017C02G1460834) （2017C02G1460834）
浙江省水产动物营养与饲料科技服务团队项目(ZJANFTST 2017-2) （ZJANFTST 2017-2）
浙江省重中之重一级学科建设项目 National Natural Science Foundation of China(41476125) （41476125）
Key Research Program of Zheji-ang Province of China(2017C02G1460834) （2017C02G1460834）
Zhejiang Aquaculture Nutrition&Feed Technology Service Team(ZJANFTST 2017-2) （ZJANFTST 2017-2）
Open Fund of Zhejiang Provincial Top Key Discipline of Aquaculture in Ningbo University （）
