水产学报2018，Vol.42Issue(3)：388-398,11.DOI:10.11964/jfc.20170210711
不同健康程度和抗生素氟苯尼考干预下斑石鲷肠道菌群的结构差异
Variance analysis of bacterial community in the intestine of cultured spotted knifejaw(Oplegnathus punctatus)at different healthy levels and intervened with florfenicol
摘要
Abstract
In order to evaluate the changes of the bacterial communities in the intestine of spotted knifejaw(Opleg-nathus punctatus)at different healthy levels and intervened with florfenicol,and to find out the relationship with environmental factors, the Illumina HiSeq PE250 high-throughput technique was used to analyze the bacterial community diversity and structure of the fish intestine for healthy, sub-healthy, typical dark body disease (DBD) infected and oral florfenicol intervened individuals, as well as the culture water and pellet feed. The results showed that bacterial diversity in the culture water was higher than that of intestine and pellet feed. The dominant bacterial communities in intestine were Proteobacteria, Firmicutes and Tenericutes, at different health levels and with florf-enicol intervention, and the proportions of corresponding operational taxonomic unit (OTU) number to all the OTU in the samples were above 85%. It could affect the ranking order of the top 20 dominant bacterial species in intest-ine when the DBD occurred,with the relative abundance of Vibrio in Proteobacteria increasing significantly.In ad-dition,the DBD symptoms aggravated gradually,with the increase of Vibrio abundance.Feeding with florfenicol could reduce the Vibrio abundance from 60.33% to 1.29% in the intestine of diseased fish,and also change its structure of bacterial community to a greater degree, which proved that florfenicol was effective in treating DBD. It was also found that the culture water and pellet feed had a certain influence on intestine bacterial community, and the effect of the culture water was higher than the pellet feed. The present results would provide a reference basis and technical support for the health culture, disease control and microecological study in spotted knifejaw.关键词
斑石鲷/黑身病/氟苯尼考/肠道菌群/高通量测序/操作分类单元(OTU)Key words
Oplegnathus punctatus/dark body disease(DBD)/florfenicol/intestine bacterial community/high-throughput technique/operational taxonomic unit (OTU)分类
农业科技引用本文复制引用
许燕,王印庚,张正,姜燕,廖梅杰,李彬,王凯,李文生..不同健康程度和抗生素氟苯尼考干预下斑石鲷肠道菌群的结构差异[J].水产学报,2018,42(3):388-398,11.基金项目
国家科技支撑计划(2012BAD17B03) （2012BAD17B03）
山东省自主创新成果转化专项(2013ZHZX2A0801)National Key Technology R&D Program of China(2012BAD17B03) （2013ZHZX2A0801）
Special Research Funds for Independent Innovation and Scientific&Technology Achievements(2013ZHZX2A0801) （2013ZHZX2A0801）
