水产学报2018，Vol.42Issue(3)：399-409,11.DOI:10.11964/jfc.20170310737
健康与患病凡纳滨对虾肠道菌群结构及功能差异研究
Characterizing the compositional and functional structures of intestinal micro-flora between healthy and diseased Litopenaeus vannamei
摘要
Abstract
To investigate the difference of the compositional and functional structures of the intestinal microflora between healthy and diseased Litopenaeus vannamei,we screened the intestinal bio-indicators to evaluate the health status of the host, and assess the functional redundancy of the intestinal microflora in response to disease. Based on the Illumina high-throughput sequencing technology, we compared the intestinal microflora between healthy and diseased L.vannamei,and analyzed the functional profiles through the Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). In addition, we estimated the correlation between the intestinal microflora structures and predicted the functional compositions. The results showed that the occurrence of disease was associated with significant changes of the bacterial community structures and functional compositions, and there was no significant change in bacterial diversity. Compared with the intestinal microflora of the healthy L.vannamei,the relative abundances of Actinobacteria,Planctomycetes and Verrucomicrobia signific-antly decreased in the diseased L.vannamei,whereas,the Gammaproteobacteria and Bacteroidetes exhibited an opposite pattern. In addition, 16 sensitive indicator families were screened, and their occurrences can indicate the health status of the host very well.Furthermore,the abundance of pathways involved in Vibrio spp.infection signi-ficantly increased in the diseased L.vannamei;in contrast,the pathways for antibacterial and immunity,such as lysosome and peroxisome pathways, markedly decreased in diseased shrimp. Notably, there was a significant and positive association in compositional and functional composition between the gut microbiota community, indicat-ing a low functional redundancy of the shrimp gut bacterial community. The occurrence of disease was associated with the change of the intestinal microflora, which subsequently disrupts the bacterial-mediated functions. Further, this study provides sensitive bio-indicators for evaluating the host health status.关键词
凡纳滨对虾/肠道菌群/功能组成/指示细菌科/功能冗余Key words
Litopenaeus vannamei/intestinal microbiota/functional composition/indicator families/functional redundancy分类
农业科技引用本文复制引用
郁维娜,戴文芳,陶震,熊金波..健康与患病凡纳滨对虾肠道菌群结构及功能差异研究[J].水产学报,2018,42(3):399-409,11.基金项目
浙江省应用公益性项目(2016C32063) （2016C32063）
宁波市科技惠民项目(2017C10044)Zhejiang Province Public Welfare Technology Application Research Project(2016C32063) （2017C10044）
Project of Science and Technology Department of Ningbo(2017C10044) （2017C10044）
