(鰤)鱼诺卡氏菌动力蛋白调节蛋白rob1/LC7的亚细胞定位和功能初步研究
Subcellular localization and preliminary function study of dynein regulation protein rob1/LC7 from Nocardia seriolae
摘要
Abstract
Fish nocardiosis is a chronic systemic granulomatous disease,and Nocardia seriolae is its main patho-gen. A dynein regulation protein rob1/LC7, which is likely to be secreted and to target host cell mitochondria, was found by the bioinformatics analysis on the whole genome sequence of N.seriolae.In order to determine the sub-cellular localization and study the preliminary function of rob1/LC7 from N.seriolae,the gene cloning,eukaryotic expression plasmid construction, secreted protein identification, subcellular localization and apoptosis detection of rob1/LC7 were carried out in this study.rob1/LC7 was successfully cloned from N.seriolae and the eukaryotic ex-pression plasmid of pEGFP- rob1/LC7 and pcDNA- rob1/LC7 were constructed. Mass spectrometry analysis of ex-tracellular products from N.seriolae confirmed that rob1/LC7 was a secreted protein.Subcellular localization of rob1/LC7-GFP fusion protein discovered that the green fluorescence was distributed in the whole cell and did not co-localize with mitochondria. In addition, apoptosis assay revealed that apoptosis was induced by the over-expres-sion of rob1/LC7 in FHM cell.rob1/LC7 from N.seriolae is a secreted protein which does not co-localize with mi-tochondria.It may help the host cell survival and immune escape of N.seriolae by participating the cell apoptosis regulation and may play an important role in the pathogenesis of N.seriolae.关键词
(鰤)鱼诺卡氏菌/动力蛋白调节蛋白rob1/LC7/分泌蛋白/亚细胞定位/细胞凋亡Key words
Nocardia seriolae/dynein regulation protein rob1/LC7/secreted protein/subcellular localization/cell apoptosis分类
农业科技引用本文复制引用
夏立群,陈锐敏,廖保山,苏泽杰,徐亮,童邦卓,黄嘉慧,鲁义善..(鰤)鱼诺卡氏菌动力蛋白调节蛋白rob1/LC7的亚细胞定位和功能初步研究[J].水产学报,2018,42(3):419-430,12.基金项目
广东省自然科学基金(2017A030313179) （2017A030313179）
广东省科技发展专项(2016A050502061) （2016A050502061）
深圳市科技计划(JCYJ20170306161613251) （JCYJ20170306161613251）
深圳大鹏新区产业发展专项(KY20160207) （KY20160207）
广东海洋大学自然科学研究项目(C13454) （C13454）
广东省大学生创新创业训练计划(201710566042)Guangdong Natural Science Foundation(2017A030313179) （201710566042）
International S&T Cooperation Projects of Guangdong Province(2016A050502061) （2016A050502061）
Shenzhen Science and Technology Project(JCYJ20170306161613251) （JCYJ20170306161613251）
Shenzhen Dapeng New District Special Fund for Industry Development(KY20160207) （KY20160207）
Natural Science Foundation of Guangdong Ocean University(C13454) （C13454）
Undergraduate Training Program for Innovation and Entrepreneurship of Guangdong Province(201710566042) （201710566042）
