水产学报2018，Vol.42Issue(3)：442-451,10.DOI:10.11964/jfc.20170110690
应用iTRAQ定量蛋白质组学技术筛选无乳链球菌鱼源株与人源株差异表达蛋白
Quantitative proteomic analysis of differential proteins in Streptococcus agalactiae piscine strain and human strain using iTRAQ
摘要
Abstract
Zebrafish and macrophages were used as in vivo and in vitro infection models to evaluate the pathogen-etic characteristics of Streptococcus agalactiae piscine strain(GD201008-001)and human strain(A909).iTRAQ and LC-MS/MS technology was used to identify differentially expressed proteins in GD201008-001 and A909, and provide new clues for exploring the pathogenic mechanism of S.galactiae strains isolated from different hosts. The pathogenetic characteristics of GD201008-001 and A909 were evaluated by analyzing LD50to zebrafish and phagocytosis of macrophages. Cellular proteins were extracted from cultures of GD201008-001 and A909, and la-belled with iTRAQ reagent. Differentially expressed proteins were identified with LC-MS/MS. The mass spectro-metry data was analyzed by Mascot2.2 and Proteome Discoverer1.4, and then subjected to biological information analysis of GO (gene ontology) and KEGG pathway. The result of pathogenicity difference analysis indicated that the virulence of GD201008-001 was higher than that of A909. Differentially expressed proteins were identified in S.galactiae piscine strain compared with human strain.A total of 368 differentially expressed proteins were re-vealed (P<0.05), among which 193 proteins were up-regulated (ratio>1.5) and 175 proteins were down-regulated (ratio<0.667) in GD201008-001. Bioinformatics analysis predicted that these proteins are mainly involved in 26 biological functions and 14 pathways. It is speculated that ClpX, GlmS and CpsIVK may play an important role in the pathogenicity of GD201008-001 and A909. These results may shed light on the pathogenic mechanism of S. galactiae strains isolated from different hosts.关键词
无乳链球菌/鱼源株/人源株/iTRAQ/蛋白质组学Key words
Streptococcus agalactiae/piscine strain/human strain/iTRAQ/proteomics分类
生物科学引用本文复制引用
郭长明,袁橙,武彩红,朱善元,刘广锦,陆承平,刘永杰..应用iTRAQ定量蛋白质组学技术筛选无乳链球菌鱼源株与人源株差异表达蛋白[J].水产学报,2018,42(3):442-451,10.基金项目
国家自然科学基金青年科学基金(31502085) （31502085）
江苏省自然科学基金青年科学基金(BK20140703) （BK20140703）
中央高校基本科研基金(KJQN201618) （KJQN201618）
江苏农牧科技职业学院科研项目(NSF201503)Youth Foundation of the National Natural Science Foundation of China(31502085) （NSF201503）
Youth Foundation of Natural Science Foundation of Jiangsu Province,China(BK20140703) （BK20140703）
Fundamental Research Funds for the Central Universities(KJQN201618) （KJQN201618）
Research Project of Jiangsu Agri-animal Husbandry Vocation-al College(NSF201503) （NSF201503）
