山东农业大学学报（自然科学版）2018，Vol.49Issue(1)：1-8,8.DOI:10.3969/j.issn.1000-2324.2018.01.001
克雷伯肺炎杆菌2,3-丁二醇合成途径功能基因研究
Study on Functional Genes of 2,3-butanediol Synthesis in Klebsiella Pneumonia
摘要
Abstract
Genes related to 2,3-butanediol synthesis form a bud operon in Klebsiella pneumoniae. BudB encodes ac etyllactate synthase and budC encodes butanediol dehydrogenase. In addition to the genes in the bud manipulation, ilvB and ilvI also encode the acetylactate synthase in the synthesis pathway of valine, and three dehydrogenases of dhaD, gldA and acyI are reported to have the activity of butanediol dehydrogenase. However, the role of these en zymes on 2,3-butanediol synthesis are not full clear. In this report, mutants of budB, ilvB and ilvI and a four gen e mutant kp-ΔbudC-ΔdhaD-ΔgldA-ΔacyI were constructed. The physical characteristics of these mutants were tested in batch fermentation. ilvB and ilvI mutation have no distinct effect to 2,3-butanediol synthesis, however, kp-Δbud B totally lost the ability to synthesis 2,3-butaneidol, it is shown that the enzyme protein of budB is the only funct ional acetylactate synthase in 2, 3-butanediol synthesis pathway. 2,3-butanediol producted by kp-ΔbudC-ΔdhaD-Δgld A-ΔacyI was decreased comparing with that of kp-ΔbudC, but it was still synthesized. The results showed that the enzyme protein encoded by dhaD, gldA and acyI was weak in the synthesis of 2, 3-butanediol, and there were m any types of alcohol dehydrogenases in the cell with the function of butanediol dehydrogenase.关键词
克雷伯肺炎杆菌/2,3-丁二醇/乙酰乳酸合成酶/丁二醇脱氢酶Key words
Klebsiella pneumoniae/2/3-butanediol/α-acetolactate synthase/butanediol dehydrogenase分类
生物科学引用本文复制引用
周吉东,郝晓蔚,王敏,史吉平,郝健..克雷伯肺炎杆菌2,3-丁二醇合成途径功能基因研究[J].山东农业大学学报（自然科学版）,2018,49(1):1-8,8.基金项目
国家自然科学基金面上项目(21576279) （21576279）
