山东农业大学学报（自然科学版）2018，Vol.49Issue(1)：14-21,8.DOI:10.3969/j.issn.1000-2324.2018.01.003
阴沟肠杆菌JP6对烟株生长及根际土壤生物学特性的影响
Effects of Enterobacter Cloacae JP6 on Tobacco Growth and Biological Characteristics of Rhizosphere Soil
摘要
Abstract
This study was aimed to research the effects of Enterobacter cloacae strain JP6 on the growth of tobacco plant, soil nutrient,enzyme activity and diversity of bacterial community under greenhouse potted conditions.Two treatments were designed: the control group was treated with 5 mL medium per plant, and the treatment group was treated with 5 mL JP6 liquid bacteria.The results showed that compared with the control group,the tobacco plant strain with 30 d of JP6 strain was significantly increased by 23.89%, and the maximum leaf surface area increased by 13.63%.The plant height of tobacco treated with JP6 for 60 days increased significantly by 8.55%;After harvest,the contents of stem and leaf total nitrogen and total phosphorus in JP6 were significantly increased.Compared with the control,the total nitrogen and available potassium in the soil treated by JP6 were reduced by 7.69% and 12.77%. The contents of available phosphorus and organic matter increased by 3.45% and 11.95% respectively.The activities of catalase,sucrase,neutral phosphatase and acid phosphatase in soil increased by 21.15%, 39.25%, 23.9% and 15.2% respectively. Illumina MiSeq high-throughput sequencing analysis showed that the application of JP6 strain affected the diversity of soil bacterial community.The bacterial species in tobacco rhizosphere soil treated with JP6 and control were 4297 ±92 and 4420 ±179 OTUs, respectively. There are 17 species of endemic bacteria in tobacco rhizosphere soil treated with JP6 application,and there were 5 bacteria in CK.Compared with the control,there were 11 bacteria relative abundance in the rhizosphere soil of JP6 than CK,among which Azotobacter and Exiguo bacterium were unique to JP6 group.The results showed that the E. cloacae strain JP6 can promote the growth of tobacco,change the soil enzyme activity and bacterial flora structure in rhizosphere soil,and increase the relative abundance of phosphate-dissolving bacteria.It is of guiding significance for the practical application of JP6 strains in agriculture.关键词
阴沟肠杆菌/烟草/促生作用/土壤酶活/细菌群落结构Key words
Enterobacter cloacae/tobacco/growth promoting effect/soil enzyme activity/bacterial community structure分类
生物科学引用本文复制引用
刘虎,易建华,周东波,周曙光,刘凯,汪城墙,丁延芹,杜秉海..阴沟肠杆菌JP6对烟株生长及根际土壤生物学特性的影响[J].山东农业大学学报（自然科学版）,2018,49(1):14-21,8.基金项目
湖南中烟工业有限责任公司科技项目(2011-yc-0002) （2011-yc-0002）
山东省重大科技专项(2015ZDXX0502B02) （2015ZDXX0502B02）
