山东农业大学学报（自然科学版）2018，Vol.49Issue(1)：90-93,4.DOI:10.3969/j.issn.1000-2324.2018.01.018
4个苹果品种的AFLP分子标记研究
Study on AFLP Molecular Marker of Four Apple Cultivars
摘要
Abstract
AFLP molecular marker technique was used for genetic analysis of 4 apple varieties.The results showed that 826 DNA fragments were amplified from 12 pairs of primers, including 595 polymorphic bands, with an average of 69 bands amplified by one pair of primers, the polymorphic percentage was 72.0%. 558 bands of Taishan Starking Delicious were amplified, including 351 polymorphic bands and 102 distinctive bands, which were significantly higher than those of other cultivars. Yimeng Short Shoot Starking Delicious and Starking Double Red Delicious amplified 517and 497 DNA bands respectively,in which polymorphic bands were 310 and 290 respectively,and distinctive bands were 64 and 55 respectively. Golden Delicious was amplified 380 bands in which 173 bands were polymorphic. The genetic relationship from other varieties is far.The variation range of genetic similarity coefficient of germplasm was 0.5846~0.7515. NTSYS2 10 was used for cluster analysis of UPGMA,which was consisted with phenotypic classification,and the AFLP marker technology system of Apple was established.关键词
品种/AFLP/分子标记Key words
Cultivar/AFLP/molecular markers分类
农业科技引用本文复制引用
王晓英,郭廷松,王新花,殷红燕,李慧,杨波,高红,边晓惠,孔风芹..4个苹果品种的AFLP分子标记研究[J].山东农业大学学报（自然科学版）,2018,49(1):90-93,4.基金项目
山东省农业良种工程项目:泰山三岔红星苹果的保护和创新利用研究(2008) （2008）
