山东中医杂志2018，Vol.37Issue(3)：237-240,4.DOI:10.16295/j.cnki.0257-358x.2018.03.019
内关电针预处理对心肌缺血再灌注损伤模型大鼠的影响
Effect of Nei Guan Electroacupuncture Preconditioning on Myocardial Ischemia Reperfusion Injury Model Rats
摘要
Abstract
Objective:To observe the effect of nei guan (PC 6) electroacupuncture (EA) preconditioning on cardiac myocytes protein kinase C(PKC),ATP-sensitive potassium channel(KATP) and mitochondrial permea-bility transition pore (MPTP) in myocardial ischemia reperfusion injury (MIRI) rats,and to explore the un-derlying mechanism for needling nei guan(PC 6) to prevent cardiovascular disease and the related meridian point zang-fu theory. Method:There were 40 male SD rats randomly divided into sham operation group , MIRI group,EA nei guan group and EA huan tiao group,10 rats in each group. Before establishing the MIRI animal model,EA nei guan group and EA huan tiao group were given EA 20 minutes per day,for 7 days. Ligation was conducted in the upper and middle 1/3 junction of the left anterior descending coronary artery to establish the animal model. After establishing the animal model ,electrocardiography was conducted. Ligating lasted for 40 minutes and was followed by reperfusion for 60 minutes. The heart tissue was sam-pled. The PKC,Kir 6.1 and MPTP were examined and the ultrastructure of cardiac myocytes was observed. Results:In MIRI group,PKC and Kir6.1 significantly decreased(P<0.01),while MPTP increased significantly (P<0.01),compared with the sham operation group. In EA nei guan group,PKC and Kir6.1 significantly in-creased(P<0.01),while MPTP significantly decreased(P<0.01),compared with MIRI group and EA huan tiao group. There was no significant difference between MIRI group and EA group(P>0.05). Conclusion:Nei guan EA preconditioning through activation of PKC,increases the opening of KATP,and inhibits the opening of MPTP,to relieve MIRI,and therefore it has a protective effect on the myocardium.关键词
心肌缺血再灌注损伤/电针/内关/心肌细胞蛋白激酶/ATP敏感性钾通道/线粒体膜通透性转换孔Key words
myocardial ischemia reperfusion injury/electroacupuncture/nei guan (PC 6)/protein kinase C/ATP sensitive potassium channels/mitochondrial permeability transition pore分类
医药卫生引用本文复制引用
冯果,王超,阳仁达,宋瑾,谭成富,刘薇薇,严洁..内关电针预处理对心肌缺血再灌注损伤模型大鼠的影响[J].山东中医杂志,2018,37(3):237-240,4.基金项目
国家自然科学基金项目(编号:81574080) （编号:81574080）
湖南省中医药管理局课题(编号:2015211) （编号:2015211）
湖南省高校创新平台开放基金项目(编号:15k093) （编号:15k093）
2015年针灸推拿学湖南省"十二五"重点学科开放基金资助项目(序号:06) （序号:06）
