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56种中药提取物对棉花黄萎病的防治效果研究

姜岚 庞金环 肖伟烈 张国丽 刘俊 杨超

生物技术通报2018,Vol.34Issue(2):128-134,7.
生物技术通报2018,Vol.34Issue(2):128-134,7.DOI:10.13560/j.cnki.biotech.bull.1985.2018-0025

56种中药提取物对棉花黄萎病的防治效果研究

Control Effects of 56 Extracts of Chinese Traditional Medicine on Cotton Wilt Disease

姜岚 1庞金环 1肖伟烈 2张国丽 3刘俊 1杨超1

作者信息

  • 1. 中国科学院微生物研究所,北京 100101
  • 2. 云南大学教育部和云南省自然资源药物化学重点实验室,昆明 650091
  • 3. 新疆农垦科学院,石河子 832000
  • 折叠

摘要

Abstract

The bacteriostatic activity and control effects of 56 extracts from Chinese traditional medicine were screened and determined in order to acquire the ingredients that may effectively suppress the mycelial growth of Verticillium dahlia and increase the control effects on cotton wilt disease. The results of growth rate showed that the inhibition rate on V. dahlia mycelial growth by 26 of all tested 56 extracts was up to 50%,which were mainly distributed in 9 medicinal plants of Schisandra chinensis(Turcz.)baill,Schisandra propinqua var. sinensis, Manglietiastrum sinicum,Euphorbia sikkimensics,etc. Further,the cotton plants were treated by the extracts and the statistics of disease index and control effect was conducted. The results explained that the chloroform :acetone(3:2,V/V)extract of S. chinensis(sampled from Heilongjiang province)presented significant control effects to cotton wilt disease. The stem dissection revealed that the extract effectively suppressed the browning of vascular bundle caused by wilt disease,and the symptom of leaf-falling from wilt disease in whole plant was significantly alleviated. The above data indicate that the chloroform :acetone(3:2,V/V)extract from S. chinensis not only effectively inhibits the growth of V. dahlia in vitro,but also induces the resistance in cotton,thus decreases the browning of vascular bundle and alleviates the occurrence of cotton wilt disease..

关键词

中药提取物/大丽轮枝菌/棉花黄萎病/防治效果

Key words

Chinese traditional medicine/Verticillium dahliae/cotton wilt disease/induced resistance

引用本文复制引用

姜岚,庞金环,肖伟烈,张国丽,刘俊,杨超..56种中药提取物对棉花黄萎病的防治效果研究[J].生物技术通报,2018,34(2):128-134,7.

基金项目

国家重点研发计划项目(2017YFD0200900) (2017YFD0200900)

生物技术通报

OA北大核心CSCDCSTPCD

1002-5464

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