山西农业大学学报（自然科学版）2018，Vol.38Issue(3)：18-22,35,6.DOI:10.13842/j.cnki.issn1671-8151.201711023
碳源和乙醇调控雨生红球藻生长与虾青素积累
Different carbon sources plus ethanol improve the growth rate and astaxanthin accumulation in Haema-tococcus pluvialis
摘要
Abstract
[Objective]The growth rate and astaxanthin content in Haematococcus pluvialis determine the economic efficiency of commercial products.The current study was conducted to develop a new method to enhance the growth rate and astaxanthin bio-synthesis in H.pluvialis.[Methods]In this study,BG-11 was used as basic medium.Gaseous carbon dioxide(CO2)and sodium acetate were selected as two carbon sources,with or without ethanol as different treatments.The effects of ethanol and carbon sources on the growth and astaxanthin biosynthesis of H.pluvialis were measured at certain time interval.[Results]Results in-dicated that the growth rate and astaxanthin biosynthesis in H.pluvialis were significantly increased with ethanol addition(P<0.05)H.pluvialis cultured in the medium of(BG-11+sodium acetate)+ethanol grew fast,and cell density was quickly reached up to the highest of 1.587×106cells·mL—1after 4 days treatment,while algae in the control group(BG-11+sodium acetate)grew slowly,and the highest cell density(1.187×106cells·mL—1)was reached on 8 days post treatment.Cell biomass and astaxanthin content in the treatment of(BG-11+sodium acetate)+ethanol were increased to 2.74 g·L—1and 3.51 %(dry weight,DW), which were significantly higher than those in control group(BG-11+sodium acetate)by 2.98 and 1.71 fold,respectively on 14-days post treatment.Similarly,H.pluvialis cultured in the medium of(BG-11+CO2)+ ethanol grew speedy to the greatest cell density of 1.236×106cells·mL—1on 6 days post treatment,followed by accumulating biomass of 2.59 g·L—1and astaxanthin content of 3.10 %(DW)on 14 days,which were both higher than that in the control group(BG-11+CO2)at 0.7 g·L—1and 1.43 %(DW),respectively.[Conclusion]The present findings provide the scientific reference for developing a simple and effec-tive system of large-scale cultivation with the addition of environment-friendly and low-cost solvent,ethanol,as a growth regulator to increase the growth rate and astaxanthin accumulation in H.pluvialis.关键词
雨生红球藻/虾青素/碳源/乙醇Key words
Haematococcus pluvialis/Astaxanthin/Carbon source/Ethanol分类
农业科技引用本文复制引用
王晓丹,程蔚兰,赵熙宁,史飞飞,杭伟,季春丽,李润植..碳源和乙醇调控雨生红球藻生长与虾青素积累[J].山西农业大学学报（自然科学版）,2018,38(3):18-22,35,6.基金项目
国家"948"项目(2014 —Z39) （2014 —Z39）
山西省煤基重点科技攻关项目(FT —2014 —01) （FT —2014 —01）
山西省重点科技项目(201603D312005) （201603D312005）
