山西农业大学学报（自然科学版）2018，Vol.38Issue(3)：52-56,5.DOI:10.13842/j.cnki.issn1671-8151.201710025
PEG胁迫下偏关苜蓿ProDH基因表达与活性分析
Gene expression level and activity of ProDH in Medicago Sativa cv.Pianguan under PEG stress
摘要
Abstract
[Objective]The aim of present study was to explore the relationship between gene expression levels ,activity of ProDH ,and proline contents in Medicago Sativ a cv .Pianguan under PEG stress .[Methods]The alfalfa were treated with PEG (20％) for 0 h ,24 h ,48 h and 72 h ,and the proline contents ,activities and expression of ProD H mRNA of the plants were determined using qRT-PCR .[Results]The proline contents and expression of ProD H were increased constantly with the increase of treating time ,while the activity of ProDH was decreased .Gene expression of ProDH was upregulated .[Conclusion]The result showed that ProDH activity was inconsistent with expression of ProD H mRNA .In conclusion ,the enzymatic activity of ProDH could be dual controlled by both transcription and translation .关键词
偏关苜蓿/PEG胁迫/ProDH基因Key words
Medicago Sativ a cv .Pianguan/PEG stress/ProD H gene分类
农业科技引用本文复制引用
贾蓉,庞妙甜,原慧,熊乙,董宽虎,朱慧森..PEG胁迫下偏关苜蓿ProDH基因表达与活性分析[J].山西农业大学学报（自然科学版）,2018,38(3):52-56,5.基金项目
国家自然科学基金(31402131) （31402131）
中国博士后科学基金(2014M561204) （2014M561204）
