实用医学杂志2018，Vol.34Issue(4)：552-555,4.DOI:10.3969/j.issn.1006-5725.2018.04.008
条件性胰岛β细胞DEPTOR基因敲除小鼠构建及鉴定
Construction and identification of conditional islet βcell DEPTOR knockout mice
摘要
Abstract
Objective To study the function of Deptor gene on the regulation of diabetes mellitus in suc-cessfully constructed and identified islet β-cell conditionally DEPTOR knockout mice model. Method By cross-breeding Deptorflox/floxmice with Cre mice expressed conditional specifically in pancreatic β-cell,Deptorflox/+Cre+/-mice were acquired and their genotypic identification was then performed. As the mice model of this study, Deptorflox/floxCre+/-mice were generated by crossing Deptorflox/+Cre+/-mice with Deptorflox/floxmice.Genotypic identifica-tion was performed by PCR at the age of 3 weeks. Tamoxifen was administered through intraperitoneal injection to induce the activation of the Cre recombination in islet beta cells of 8 weeks mice.Double immunofluorescence label-ing was then applied to identify the knockout effect of DEPTOR gene. Results Ten Islets Deptor knockout mice models were successfully acquired after 10-month cross-breeding. Validated genotype by PCR analysis were Deptorflox/floxCre+/- and double immunofluorescence labeling showed a significant difference between knockout mice and rodent controls. Conclusion Our study successfully constructs the islets conditionally Deptor deleted mice model by using Cre-loxp recombination system,providing a promising appliable animal model for study of dia-betes mellitus pathogenetic mechanism.关键词
DEPTOR/Cre/loxp系统/胰岛β细胞Key words
DEPTOR/Cre/loxp recombination system/pancreatic β cell引用本文复制引用
赖舒畅,邱鸿,王肖,潘道延,王桢钰,李凯,白晓春,沈洁..条件性胰岛β细胞DEPTOR基因敲除小鼠构建及鉴定[J].实用医学杂志,2018,34(4):552-555,4.基金项目
广州市科技计划项目(编号:201508020262) （编号:201508020262）
