天津科技大学学报2018，Vol.33Issue(1)：14-19,6.DOI:10.13364/j.issn.1672-6510.20160174
紫红红球菌9α-羟化酶loop对酶活性的影响
Influence of Loop on Rhodococcus rhodochrous 9α-Hydroxylase Activity
摘要
Abstract
3-ketosteroid-9α-hydroxylase is the key enzyme that transforms androst-4-ene-3,17-dione into 9α-hydroxyan-drost-4-ene-3,17-dione.There exists a flexible loop which consists of 16 amino acids in front of the entrance of the active center of its oxygenase component.To understand the influence of the loop on the enzyme activity,site-directed mutagenesis was used to investigate the amino acids of the loop which moved remarkably in the whole protein structure.As a result, T224,N225 and Y226 were learned to adjust the substrate channel and transport the substrate with their surroundingα5-helix and β-sheet amino acids,while D227 and D228 played cerlain roles in supporting and fixing the loop,allowing T224-Y226 to interact with their surrounding amino acids.This work provided theoretical foundations for further research on 9α-hydroxylase modification through protein engineering.关键词
3-甾酮-9α-羟化酶/雄甾-4-烯-3,17-二酮/9α-羟基雄甾-4-烯-3,17-二酮/loop/定点突变Key words
3-ketosteroid-9α-hydroxylase/androst-4-ene-3/17-dione/9α-hydroxyandrost-4-ene-3/17-dione/loop/site-directed mutagenesis分类
生物科学引用本文复制引用
刘扬,申雁冰,王九彬,王敏..紫红红球菌9α-羟化酶loop对酶活性的影响[J].天津科技大学学报,2018,33(1):14-19,6.基金项目
国家自然科学基金资助项目(21276196,21406167) （21276196,21406167）
