天津科技大学学报2018，Vol.33Issue(1)：25-29,5.DOI:10.13364/j.issn.1672-6510.20160192
潘糖标准品的制备与应用可行性评价
Preparation of Standard Panose and Evaluation of its Applicability
李玮 1董自星 2李普均 1金鹏 2刘晓光 2路福平 1王正祥2
作者信息
- 1. 工业发酵微生物教育部重点实验室,天津科技大学生物工程学院,天津 300457
- 2. 天津科技大学化工与材料学院,天津 300457
- 折叠
摘要
Abstract
Panose,the key component of isomaltooligosaccharides(IMO),is of great scientific and practical interest in many fields.However,it is very expensive and can not be easily bought in the market,which restricts the development and application of IMO.In this study,high performance liquid chromatography-evaporative light scattering detection(HPLC-ELSD)was adopted to qualitatively analyze all components in IMO samples and identify the functional ingredients involved.Subsequently,high performance liquid chromatography-refractive index detection(HPLC-RID)was used to sepa-rate and purify panose from IMO samples.The purity,average recovery ratio,relative standard deviations(RSDs)for repeti-tive and precision experiments of the panose obtained were determined to be(98.43±0.25)%,,(96.20±0.73)%,,0.25%,and 0.27%,,respectively.Besides,the purified panose could be kept at 2-8,℃ for more than 12,months.Therefore,the panose prepared in this study complied with Pharmacopoeia of People's Republic of China(2015,Edition)and could be used as sec-ondary standard.Knowledge gained here provides a fundamental basis for the in-depth study of IMO.关键词
低聚异麦芽糖/潘糖/分离纯化/标准品/制备Key words
isomaltooligosaccharides/panose/separation and purification/standard/preparation分类
轻工纺织引用本文复制引用
李玮,董自星,李普均,金鹏,刘晓光,路福平,王正祥..潘糖标准品的制备与应用可行性评价[J].天津科技大学学报,2018,33(1):25-29,5.
