天津中医药2018，Vol.35Issue(3)：225-229,5.DOI:10.11656/j.issn.1672-1519.2018.03.19
哈氏蜈蚣线粒体基因组全序列分析
Analysis of mitochondrial genome of Scolopendra subspinipes dehaani
摘要
Abstract
[Objective]To investigate the complete mitochondrial genome of Scolopendra subspinipes dehaani. [Methods]Mitochondrial DNA of Scolopendra subspinipes dehaani was purified by differential centrifugation and rolling circle amplification.The Illumina Hiseq X Ten technique was chosen to sequence the purified mitochondrial DNA,and then the strategy of de novo assembly and mapping versu mtgenome of closely related species by MIRA 4.0 was adopted to get the complete mitochondrial sequence.This genome was annotated by Online Server MITOS and mtgenome of closely related species. [Results]The 14 538 bp complete mitochondrial genome of Scolopendra subspinipes dehaani with high coverage contains 20 transfer RNA,13 protein coding genes,2 ribosomal RNA genes(16S rRNA,RS rRNA). [Conclusion]The mtgenome of Scolopendra subspinipes dehaani was obtained,and could be further applied in the resources survey and protection of medical centipede.关键词
哈氏蜈蚣/线粒体基因组/线粒体DNA/序列分析Key words
Scolopendra subspinipes dehaani/mitochondrial genome/mitochondrial DNA/sequence analysis分类
医药卫生引用本文复制引用
孙丽冉,齐迎菊,田晓轩..哈氏蜈蚣线粒体基因组全序列分析[J].天津中医药,2018,35(3):225-229,5.基金项目
横向课题:疏血通注射液基原研究(HX2017-18). （HX2017-18）
