中国组织工程研究2018，Vol.22Issue(4)：529-534,6.DOI:10.3969/j.issn.2095-4344.0085
甘草黄酮对运动大鼠股四头肌病理学变化及FAT/CD36表达的影响
Effects of Glycyrrhiza Flavone on the pathological changes of quadriceps and expression of fatty acid translocase/CD36 in training rats
摘要
Abstract
BACKGROUND: Fatty acid translocase/CD36 (FAT/CD36) in skeletal muscle has been proved to induce skeletal muscle contraction transport to the outer membrane stimulated by movement, which increases FAT/CD36 content in cellular membrane, further leading to more fatty acids into skeletal muscle cells. OBJECTIVE: To observe the effects of Glycyrrhiza Flavone administration before exercise on the pathological changes of quadriceps and FAT/CD36 in rats. METHODS: Thirty male Sprague-Dawley rats were randomly divided into quiet, exercise and combination groups. The rats in the combination group were administrated with 10 mL/(kg?d) Glycyrrhiza Flavone, while the other two groups were given the same volume of normal saline. The rats in the exercise and combination groups were subjected to 6-week training, 19.3 m/minute at the treadmill slope of 5°. The pathological changes of quadriceps were observed by hematoxylin-eosin staining, and the expression level of FAT/CD36 was detected by immunohistochemistry and Western blot assay. RESULTS AND CONCLUSION: Under light microscope, swollen, ruptured and necrotic muscle fibers in the quadriceps were found in the excise group. In the combination group, muscle fibers tightly arranged in the quadriceps with complete edge, which had no significant difference compared with the quiet group. Immunohistochemistry showed that the expression level of FAT/CD36 in the exercise group was significantly higher than that in the quiet group (P < 0.05); the expression level of FAT/CD36 in the combination group was slightly higher than that in the quiet group, but significnatly lower than that in the exercise group (P < 0.05), all above findings were consistent with the western blot assay. Our results indicate that exercise can induce fatty acid transport and accumulation. Due to the synergistic action of Glycyrrhiza Flavonoids, FAT/CD36 increases the efficiency of fatty acid transport, thereby maintaining lipid metabolism balance in the skeletal muscle, which avoids lipid metabolic disorders.关键词
甘草黄酮/耐力训练/FAT/CD36分类
医药卫生引用本文复制引用
王兆锋,宫明明,陈家群,莫伟彬..甘草黄酮对运动大鼠股四头肌病理学变化及FAT/CD36表达的影响[J].中国组织工程研究,2018,22(4):529-534,6.基金项目
广西自然科学资金面上项目(2015GXNSFAA139186) （2015GXNSFAA139186）
广西高校科研项目(YB2014377) （YB2014377）
"省部共建药用资源化学与药物分子工程国家重点实验室"开放课题(CMEMR2012-B04) （CMEMR2012-B04）
the Natural Science Foundation of Guangxi Zhuang Autonomous Region,No.2015GXNSFAA139186 （）
the Research Project of Guangxi Universities,No.YB2014377 （）
the Open Project of the State Key Laboratory of Ministry-Province Medicinal Resources Chemistry and Drug Molecular Engineering,No.CMEMR2012-B04 （）
