中国组织工程研究2018，Vol.22Issue(4)：576-581,6.DOI:10.3969/j.issn.2095-4344.0093
针刺"足三里"穴对脾虚证模型大鼠肠系膜淋巴结T淋巴细胞亚群的影响
Effect of acupuncture at Zusanli on T Iymphocyte subsets in mesenteric lymph nodes of spleen deficiency syndrome rats
摘要
Abstract
BACKGROUND: Intestinal and lymphoid tissues constitute an important part of intestinal immunity, which plays an important regulatory role in spleen deficiency and hydronephrosis. OBJECTIVE: To observe the effect of acupuncture on T lymphocyte subsets in lymph nodes of rats with spleen deficiency, and to investigate the correlation of spleen deficiency with intestinal immunity and the mechanism of acupuncture for spleen deficiency syndrome. METHODS: Thirty-six female Sprague-Dawley rats were randomly divided into three groups: model, acupuncture and blank control groups. The rat model of spleen deficiency was established by fatigue-induced spleen injury plus abnormal diet for 31 days. Afterwards, the rats in the acupuncture group received acupuncture at Zusanli(ST 36).Urine D-xylose excretion rate was detected during modeling and treatment.Then, the mesenteric lymph nodes were removed, and the changes in T lymphocyte subsets in the mesentericlymph nodes were observed by immunohistochemistry. RESULTS AND CONCLUSION: Urine D-xylose excretion rate under spleen deficiency in the modeling and acupuncture groups was significantly lower than that in the blank control group (P < 0.05 or P < 0.01); after acupuncture, the urine D-xylose excretion rate was significantly increased compared with the modeling group (P < 0.01), but still lower than that in the blank control group (P < 0.05). The count of CD4+T lymphocytes, count of CD8+T lymphocytes and ratio of CD4+/CD8+T lymphocytes were ranked as follows: blank control group >acupuncture group>modeling group(P<0.01 or P<0.05).These results suggest that acupuncture at Zusanli can improve the urine D-xylose excretion rate, regulate the balance of T lymphocyte subsets in mesenteric lymph node of rats with spleen deficiency, thus improving the intestinal immune function, spleen deficiency systems, disorder of intestinal digestive function, intestinal digestion and absorption, as well as anorexia, loose stool, diarrhea and other symptoms of the digestive system.关键词
脾虚/针刺/肠系膜淋巴结/T淋巴细胞亚群/肠道免疫/组织构建分类
医药卫生引用本文复制引用
曾荣华,周露,欧阳厚淦,彭珊,高书亮,汪建民,吴慧婷,欧阳彦楚,崔田田..针刺"足三里"穴对脾虚证模型大鼠肠系膜淋巴结T淋巴细胞亚群的影响[J].中国组织工程研究,2018,22(4):576-581,6.基金项目
国家自然科学基金项目(81360601) （81360601）
江西省卫计委中医药科研计划项目(2014A075) （2014A075）
江西中医药大学校级课题(2013ZR0046) （2013ZR0046）
the National Natural Science Foundation of China,No.81360601 （）
the Chinese Medicine Research Program of Health and Family Planning Department of Jiangxi Province,No.2014A075 （）
the Project of Jiangxi University of Traditional Chinese Medicine,No.2013ZR0046 （）
