中国组织工程研究2018，Vol.22Issue(5)：675-679,5.DOI:10.3969/j.issn.2095-4344.0434
透明质酸水凝胶包裹骨髓间充质干细胞改善大鼠心肌梗死后心功能
Combination of hyaluronic acid and bone marrow mesenchymal stem cells promotes cardiac function after myocardial infarction
商青青 1周建业 2李凯 1孙嘉康 1孟健 1李君1
作者信息
- 1. 中国医学科学院,北京协和医学院,国家心血管病中心,阜外心血管病医院,心血管疾病国家重点实验室,北京市 100037
- 2. 滨州医学院附属医院疼痛科,山东省滨州市 256603
- 折叠
摘要
Abstract
BACKGROUND: The poor retention and survival of donor cells implanted into the myocardium limit the efficacy of cell therapy for myocardial infarction. Embedding cells in natural or synthetic biomaterials is a strategy to address this issue. OBJECTIVE: To explore the effects of bone marrow mesenchymal stem cells (BMSCs) encapsulated in hyaluronic acid (HA) hydrogel on cardiac function after myocardial infarction. METHODS:BMSCs from male Sprague-Dawley rats were isolated and cultured,and then HA-encapsulated BMSCs were cultured in vitro in the three-dimensional manner. A model of myocardial infarction was made by cutting the anterior descending artery of female Sprague-Dawley rats. After 1 week, the model rats were screened by ultrasonic testing and then eligible ones were randomly divided into four groups: PBS group (n=8), HA group (n=8), BMSCs group (n=29), and HA-encapsulated BMSCs group (n=29). At 1 week after modeling, the model rats underwent the secondary thoracotomy and the implants were injected into the marginal zone and infarcted region in corresponding groups. The survival rate and apoptosis of implanted cells were examined at post-injection day 1, week 1 and week 2 by RT-PCR and TUNEL respectively. At post-injection week 4, changes of cardiac microstructure and function were evaluated by histological examination and echocardiography. RESULTS AND CONCLUSION: Compared with the BMSCs group, HA hydrogel significantly enhanced the survival rate and reduced the apoptotic rate of BMSCs at post-injection day 1 and week 2 (both P < 0.05). At post-injection week 4, the HA+BMSCs combined treatment yielded the best recovery of cardiac function (P < 0.05). To conclude, HA hydrogel can act as a vehicle for BMSCs delivery and improve the beneficial effects of implanted BMSCs in early myocardial repair(within 2 weeks after infarction)via enhancing cell retention and survival.关键词
透明质酸/骨髓间充质干细胞/心肌梗死/细胞移植/心功能/干细胞分类
医药卫生引用本文复制引用
商青青,周建业,李凯,孙嘉康,孟健,李君..透明质酸水凝胶包裹骨髓间充质干细胞改善大鼠心肌梗死后心功能[J].中国组织工程研究,2018,22(5):675-679,5.
