中国组织工程研究2018，Vol.22Issue(5)：729-735,7.DOI:10.3969/j.issn.2095-4344.0443
雷公藤多糖诱导胰腺干细胞成胰岛样细胞团
Tripterygium wilfordii polysaccharide induces differentiation of pancreatic stem cells into islet-like cell clusters
摘要
Abstract
BACKGROUND: Inadequate sources of islet cells mean that islet cell transplantation for diabetes cannot meet the clinical demand.Therefore,in vitro induction of pancreatic stem cells to differentiate into islets has become a focus of research. OBJECTIVE:To study the effect of Tripterygium wilfordii polysaccharides on the differentiation of pancreatic stem cells from islets in mice, so as to explore the effect of traditional Chinese medicine on the differentiation of pancreatic stem cells into pancreatic beta cells. METHODS:Tripterygium wilfordii polysaccharide was used to induce the differentiation of purified mouse pancreatic stem cells into islets in vitro.The islet-like cell clusters then underwent morphologic observation, dithizone (DTZ) staining, and western blot analysis. RESULTS AND CONCLUSION: Cell morphology, cell growth characteristics and immunocytochemical staining showed that mouse pancreatic stem cells were obtained.They were induced by Tripterygium wilfordii polysaccharide into spherical islet-like structures, which had a spindly pedicle connected with the bottom of the culture flask, and were DTZ-stained to iron red. Western blot assay detected β-cytokine proteins in the islet-like cell clusters. These findings confirm that mouse pancreatic stem cells can be induced to differentiate into islet-like cell clusters containing β cells in vitro by Tripterygium wilfordii polysaccharide.关键词
雷公藤多糖/胰腺干细胞/形态学/生长特征/诱导分化/胰岛样细胞团/免疫细胞化学染色/双硫腙/Western-blot/β细胞素/组织工程分类
医药卫生引用本文复制引用
岑妍慧,石磊,李中华,贾微,杨瑞,包鹃,何国珍,吴晓君,钟静,邓慧枫..雷公藤多糖诱导胰腺干细胞成胰岛样细胞团[J].中国组织工程研究,2018,22(5):729-735,7.基金项目
国家自然科学基金项目(81503406) （81503406）
中国博士后科学基金(2017M6232) （2017M6232）
广西壮族自治区卫生厅中医药科技专项课题(GZBZ16-07,GZLC16-23) （GZBZ16-07,GZLC16-23）
广西自然科学基金(2014GXNSFAA118177)the National Natural Science Foundation of China,No.81503406 （2014GXNSFAA118177）
the China Postdoctoral Science Foundation,No.2017M6232 （）
the Special Fund for Traditional Chinese Medicine by the Health Department of Guangxi Zhuang Autonomous Region,No.GZBZ16-07,GZLC16-23 （）
the Natural Science Foundation of Guangxi Zhuang Autonomous Region,No.2014GXNSFAA118177 （）
