畜牧兽医学报2017，Vol.48Issue(11)：2046-2058,13.DOI:10.11843/j.issn.0366-6964.2017.11.005
SLA-DOB及CD4基因新突变显著影响长白妊娠母猪的血常规性状
Novel Genetic Mutations in Porcine SLA-DOB and CD4 Significantly Affect the Routine Blood Traits of Pregnant Landrace Sows
摘要
Abstract
This study aimed to find single nucleotide polymorphism (SNP) sites and molecular markers affecting the routine blood traits of pregnant sows.Porcine SLA-DOB and CD4 were chosen as candidate genes,blood samples were randomly collected from 358 pregnant Landrace sows and used to detect 19 routine blood indexes,then association analysis was conducted between the polymorphic sites of candidate genes and the routine blood traits.We found that 12 mutations,in which SNP1,SNP2 and SNP3 of SLA-DOB gene as well as Ins9,SNP10 and SNP11 in CD4 gene were all newly discovered mutations.SNP1 was significantly associated with mean platelet volume (MPV) (P＜0.01),hematocrit (HCT) and red cell volume distribution width SD (RDW_SD) (P＜0.05);SNP2 was extremely significantly associated with MPV (P＜0.01);SNP3 was significantly associated with mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) (P＜0.05);Ins9 was significantly associated with mean corpuscular volume (MCV) (P＜0.05) and MPV (P＜0.01);SNP10 and SNP11 were significantly associated with MCV,MCH,MPV (P＜0.01) and RDW_SD (P＜0.05);meanwhile,SNP10 was significantly associated with MCHC (P＜0.05).These novel mutations are potential important polymorphic loci affecting the routine blood traits in pregnant sows.The results suggest that the SLA-DOB and CD4 genes as well as their genetic mutations can be as important candidate genes and key molecular markers affecting immunity of pregnant sows.关键词
妊娠母猪/血常规性状/SLA-DOB/CD4/关联分析Key words
pregnant sows/routine blood traits/SLA-DOB/CD4/association analysis分类
农业科技引用本文复制引用
张丰霞,杨婷,敖红,谭振,邢凯,翟丽维,俞英,王楚端..SLA-DOB及CD4基因新突变显著影响长白妊娠母猪的血常规性状[J].畜牧兽医学报,2017,48(11):2046-2058,13.基金项目
北京市现代农业产业技术创新团队(25019126) （25019126）
国家自然科学基金项目(31572361) （31572361）
国家生猪产业技术体系(CARS-35) （CARS-35）
长江学者与创新团队发展计划(IRT1191) （IRT1191）
