畜牧兽医学报2017，Vol.48Issue(11)：2059-2067,9.DOI:10.11843/j.issn.0366-6964.2017.11.006
miR-132-3p靶向UCP2调节绵羊前体脂肪细胞分化的研究
Study on the Regulation of miR-132-3p in Ovine Preadipocyte Differentiation by Targeting UCP2
摘要
Abstract
This study aimed to explore the regulatory mechanism of miR-132-3p targeting UCP2 expression in ovine preadipocytes.In this study,ovine subcutaneous fat cells were used to investigate the mechanism of miR-132-3p targeting UCP2 in ovine preadipocytes.The binding sites of miR-132-3p to UCP2 were predicted by bioinformatics softwares,and were verified via double luciferase report system.Expression of UCP2 mRNA and protein were detected by RT-qPCR and Western blotting,respectively after overexpressing miR-132-3p.The expression of UCP2 mRNA and miR-132-3p were detected by RT-qPCR during differentiation stage in ovine preadipocyte.The results showed that miR-132-3p had binding site to UCP2 3'-UTR and significantly downregulated the relative fluorescence activity of recombinant double fluorescent plasmid(P＜0.05).Overexpressing miR-132-3p significantly down-regulated both UCP2 mRNA(P＜0.05) and protein expressions.At last,we found a negative correlation between the expression of miR-132-3p and UCP2 at the middle stage during ovine preadipocytes differentiation.In conclusion,miR-132-3p inhibits UCP2 expression at both levels of mRNA and protein by specifically binding to UCP2 3'-UTR,and thus promotes ovine preadipocytes differentiation to a certain extent.关键词
绵羊/前体脂肪细胞/细胞分化/miR-132-3p/UCP2Key words
sheep/preadipocytes/cell differentiation/miR-132-3p/UCP2分类
农业科技引用本文复制引用
师涛,闫晓茹,潘洋洋,景炅婕,乔利英,郭云利,申彩云,张富权,刘文忠..miR-132-3p靶向UCP2调节绵羊前体脂肪细胞分化的研究[J].畜牧兽医学报,2017,48(11):2059-2067,9.基金项目
国家自然科学基金(31372292) （31372292）
山西省优势肉用家畜高效安全生产协同创新中心项目 （）
