畜牧兽医学报2017，Vol.48Issue(11)：2076-2083,8.DOI:10.11843/j.issn.0366-6964.2017.11.008
miR-130b靶向PPARγ抑制家兔前体脂肪细胞分化
miR-130b Inhibits the Differentiation of Rabbit Preadipocytes by Targeting PPARγ
摘要
Abstract
The aim of this study was to demonstrate that miR-130b could target PPARγ to inhibit the differentiation of rabbit preadipocytes.Primary perirenal preadipocytes of New Zealand young rabbits were cultured and induced differentiation by cocktail as in vitro experimental model,and then the expression of miR-130b,PPARγ and C/EBPα at 0,2,6,10 d during differentiation were determined by RT-qPCR.Bioinformatics softwares were used to identify homology and predict target genes of miR-130b;The preadipocytes were transfected with miR-130b mimic and miR-130b inhibitor in vitro,and the expression of miR-130b,PPARγ and C/EBPα were detected.The preadipocytcs were stained with Oil Red O.The results showed that:1) In the process of preadipocyte differentiation,the expression of miR-130b,PPARγ and C/EBPα were variant;There were a large orange red lipid droplets with Oil Red O staining in the preadipocytes differentiated for 8 days;Bioinformatics software predicted that PPARγ could be used as the target gene of miR-130b,and the sequence of miR-130b had a high homology in mammals.2) After overexpression of miR-130b,the expression of PPARγ and C/EBPα in mimic group were very significantly lower than that in NC group (P＜0.01).3) After knockdowned miR-130b,the expression of PPARγ in inhibitor group was very significantly higher than that in inhibitor NC group (P＜ 0.01),the expression of C/EBPα in inhibitor group was significantly higher than that in inhibitor NC group (P＜0.05).4) Oil Red O staining showed that the degree of differentiation.:inhibitor group＞NC group＞mimic group.miR-130b is differentially expressed in the differentiation process of rabbit preadipocytes and may inhibit the differentiation of preadipocytes by targeting PPARγ.关键词
miR-130b/家兔/前体脂肪细胞/PPARγKey words
miR-130b/rabbit/preadipocytes/PPARγ分类
农业科技引用本文复制引用
何洪炳,蔡明成,梁小虎,贾先波,赖松家..miR-130b靶向PPARγ抑制家兔前体脂肪细胞分化[J].畜牧兽医学报,2017,48(11):2076-2083,8.基金项目
国家兔产业技术体系(CARS44-A-2) （CARS44-A-2）
优质特色兔配套系选育与育种材料创新(2016NYZ0046) （2016NYZ0046）
