胰岛素和FSH对体外培养猪卵泡颗粒细胞雌激素的影响OA北大核心CSCDCSTPCD
Effects of Insulin and FSH on E2 Production of Pig Ovarian Follicular Granulosa Cells in vitro Culture
旨在研究胰岛素(Insulin)和促卵泡素(Follicular stimulating hormone,FSH)对猪卵泡发育过程的影响.猪屠宰后收集卵巢,选择健康卵泡并分离颗粒细胞(Granulesa cells,GCs),设定对照组和重复组,胰岛素、FSH浓度梯度下Long-term体外培养,7d后显微镜观察细胞增殖情况并计数,竞争法测定雌激素(E2)浓度.数据分析表明:当FSH为0 ng· mL-1时,随着胰岛素浓度的增加,猪卵泡GCs细胞…查看全部>>
To research the effects of insulin and FSH on porcine ovarian follicular development,ovaries were collected and follicular granulosa cells (GCs) were isolated.Setting control groups and repetitive groups,GCs were cultured for 7 d treated with different concentrations of insulin and FSH by long-term culture in vitro.Observing the growth situation and counting the numbers of GCs in each well.Competition method was performed to detect the E2 concentrations in m…查看全部>>
李鹏飞;孟金柱;郝庆玲;毕锡麟;王锴;朱芷葳;吕丽华
山西农业大学生命科学学院,太谷030801铜仁学院乌江学院,铜仁554300山西农业大学生命科学学院,太谷030801山西农业大学动物科技学院,太谷030801山西农业大学动物科技学院,太谷030801山西农业大学生命科学学院,太谷030801山西农业大学动物科技学院,太谷030801
农业科技
猪颗粒细胞胰岛素促卵泡素雌激素体外培养
piggranulosa cellinsulinFSHE2in vitro culture
《畜牧兽医学报》 2017 (11)
miRNA互作对于小鼠皮肤黑色素细胞中Mitf基因的调节机制
2084-2090,7
国家自然科学基金(31402156)山西省回国留学人员科研资助项目(2014-重点5)山西省国际科技合作项目(201603D421006)山西省三晋学者和人才引进项目山西农业大学创新基金项目(zdpy201403/201503)引进人才博士科研启动基金(2014ZZ04)青年拔尖创新人才支持计划(TYIT201403)山西省重点研发计划(一般)农业项目(201703D221020-1)
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