畜牧兽医学报2017，Vol.48Issue(11)：2157-2165,9.DOI:10.11843/j.issn.0366-6964.2017.11.017
四川省山羊源沙门菌血清型鉴定及毒力基因的分布
Distribution of Serovars and Virulence Genes in Salmonella Isolates from Goats in Sichuan Province
摘要
Abstract
The aim of this study was to investigate the serovars and virulence genes as well as the pathogenicity of Salmonella isolates from goats in Sichuan Province,which will provide the information for food safety.The 42 Salmonella strains from goats were identified by the mono-specific serum,and the carrying rates of 15 virulence genes,including invA,sopE,orgA,avrA,ttrB,sseL,rhuM,mgtC,orfL,sopB,pipD,sodC1,lpfC,pefA and spvC,were detected by PCR.According to the results of serovars and virulence genes,6 of the Salmonella strains were selected and studied the pathogenicity in BALB/c mice by oral infection.The serotyping results exhibited that the 42 Salmonella isolates were divide into 4 different serovars,incuding S.Blegdam (23/42),S.Enterica (15/42),S.Agona (3/42) and S.Newport (1/42).In 42 Salmonella strains,all isolates carried the five virulence genes of invA,orgA,pipD,sopB and lpfC.The carrying rates of the virulence genes of sopE,sseL,ttrB,rhuM,sodC1,pefA,spvC,avrA,mgtC and orfL were 97.6％,97.6％,88.1％,95.2％,92.9％,47.6％,47.6％,40.5％,40.5％ and 38.1％,respectively.Each strain carried at least 6 virulence genes,and two S.Agona strains carried virulence plasmid.There were two of S.enteritidis strains,two of S.Blegdam strains and two of S.Agona strains carrying 15 virulence genes,which could killed the no-antibiotics treated mice at the doses of 5 × 108 CFU.Furthermore,the LD50 of the S.enteritidis strain SWUN3816 was 1.6 × 102 CFU to mice.Both S.Blegdam and S.Enterica were dominant serovars from goats in Sichuan Province,and it was first to identify the S.Agona strain carrying virulence plasmid.关键词
山羊/沙门菌/血清型/毒力基因/毒力质粒Key words
goat/Salmonella/serovar/virulence gene/virulence plasmid分类
农业科技引用本文复制引用
夏宾雁,王晶晶,汤承,谭烁,冯帆,陈极奎,岳华..四川省山羊源沙门菌血清型鉴定及毒力基因的分布[J].畜牧兽医学报,2017,48(11):2157-2165,9.基金项目
国家重点研发计划课题(2016YFD0500907) （2016YFD0500907）
