畜牧兽医学报2017，Vol.48Issue(11)：2166-2172,7.DOI:10.11843/j.issn.0366-6964.2017.11.018
噬菌体RZⅢ-3的生物学特性分析及对鸡大肠杆菌的疗效
Biological Characteristics Analysis of Phage RZ Ⅲ-3 to Escherichia coli and the Therapeutic Effect on Chicken
摘要
Abstract
To solve the antibiotic resistance and find substitution of antibiotics,this study explored the basic biological characteristics of Escherichia coli phage RZⅢ-3 and its therapeutic effect on SPF chicken colibacillosis.The biological characteristics of Escherichia coli phage RZⅢ-3 showed that the titer was 109 pfu · mL-1,high stability to heat,the bacteriophage titer can reach more than 107 pfu · mL-1 after incubation at 60 ℃C for 1 h.It had a wide adaptive range of pH,and maintained a high cleavage activity at pH 4-10.The optimum pH of the phage was about 7.0,its optimal multiplicity of infection (MOI) was 0.1,and the incubation period of infected host bacteria was about 20 min.The results of transmission electron microscopy showed that the phage belonged to the T4 phage.Escherichia coli phage RZⅢ-3 on Escherichia coli 1019 had a strong ability to crack,The number of Escherichia coli 1019 in SPF chickens can be reduced by 96.5％.This indicated that the Escherichia coli phage RZⅢ-3 has a greater potential for the treatment of specific Escherichia coli infection.关键词
大肠杆菌/噬菌体/生物学特性/治疗Key words
Escherichia coli/phage/biological characteristics/therapy分类
生物科学引用本文复制引用
王杰,李璐璐,张庆..噬菌体RZⅢ-3的生物学特性分析及对鸡大肠杆菌的疗效[J].畜牧兽医学报,2017,48(11):2166-2172,7.基金项目
国家自然科学基金(81271886) （81271886）
山东省农业重大应用技术创新项目(SDAIT-11-09) （SDAIT-11-09）
山东省自然科学培养基金(ZR2014CP021) （ZR2014CP021）
