畜牧兽医学报2017，Vol.48Issue(11)：2198-2204,7.DOI:10.11843/j.issn.0366-6964.2017.11.022
保育猪舍不同粒径悬浮颗粒物细菌群落组成的初步研究
Preliminary Study on Bacterial Community Composition of Different Particle Size of Suspended Particulates in Weaning Pig House
摘要
Abstract
Bacterial community composition of different particle size of suspended particulates in weaning pig house was preliminary studied,by high-throughput sequencing.This study collected aerosol microbe in weaning pig house with 5 ％ of the male sheep blood-AGAR culture medium for sampling medium,using Andersen-level 6 impact type air microorganism sample collector.The collected aerosol microbe was trained after 24 hours to extract DNA and amplify of the V3-V4 viriable regions of 16S rDNA.Afterwords,we analyzed bacterial community composition of different particle size of suspension particles in weaning pig house,by iIIumina MiSeq high-throughput sequencing.We obtained 301 065 high quality series at all levels and identified 2 104 OTUs (operational taxonomical unit) based on the 97 ％ similarity rule;By alpha diversity analysis,flora richness from big to small,in turn,was 3_4s,3_2s,3_1s,3_6s,3_5s and 3_3s,respectively.Flora diversity was slightly different through the analysis of the different index,but the highest diversity was from 3_2s,3_5s,the lowest was from 3_3s;Flora composition was mainly composed of 4 phylum,40 species.The richness of Proteobacteria and Firmicutes were the highest.Total percentage of Acinetobacter,Comamonas,Staphylococcus and Myroides were higher than others.Flora similarity of 3_1s,3_5s,3_4s and 3_2s were high through the PCoA (principal component analysis) based on UniFrac analysis.Difference of flora structure between 3_6s,3_3s and the others was observed.The richness of bacterial flora which diameter of suspended particulate was between 2.1 and 3.3μm was the highest.Fourteen species of bacteria had been reported pathogenic to people and animals.Community structure was different at all levels,and difference of adjacent level was great.关键词
保育猪舍/悬浮颗粒物/细菌群落组成/高通量测序Key words
weaning pig house/suspended particulate/bacterial community composition/high-throughput sequencing分类
农业科技引用本文复制引用
鞠雷,郭洪梅,朱术会,李丛丛..保育猪舍不同粒径悬浮颗粒物细菌群落组成的初步研究[J].畜牧兽医学报,2017,48(11):2198-2204,7.基金项目
山东省现代农业产业技术体系生猪创新团队生产与环境控制岗位(SDAIT-08-09) （SDAIT-08-09）
潍坊市科技发展计划(2016GX045) （2016GX045）
