中国动物检疫2018，Vol.35Issue(3)：63-66,4.DOI:10.3969/j.issn.1005-944X.2018.03.018
羊传染性脓疱病毒PCR检测方法的建立与应用
Establishment and Application of a PCR Method for Detection of Contagious Pustular Dermatitis Virus
摘要
Abstract
In order to rapidly detect the Contagious pustular dermatitis virus(CPDV)in clinical practice,a PCR method was developed. According to the published gene sequence of virus in GenBank,a pair of specific primers were designed and synthesized by comparing the conserved regions of B2L sequences of multiple strains. Then the CPDV was detected by the developed PCR method,and the specificity,sensitivity and clinical application were tested. The results showed that the optimum annealing temperature was 56 ℃,the optimum amount of primer was 0.6 μL(20 μmol/L), and the detection limit was 1 pg of DNA. In addition,the method could distinguish CPDV and other viruses containing Goat pox virus,Foot and mouth disease virus,Peste des petits ruminants virus and Blue Tongue virus. As a result, a PCR method with high specificity and strong sensitivity was established,and it could be used in rapid detection of CPDV.关键词
羊传染性脓疱病毒/PCR/检测Key words
Contagious pustular dermatitis virus/PCR/detection分类
农业科技引用本文复制引用
沙娜瓦尔·塔希,买买提艾力·斯迪克,李舵,王文,美热木古丽·巴依待拉提,马晓燕,苏晓慧,盛卓君..羊传染性脓疱病毒PCR检测方法的建立与应用[J].中国动物检疫,2018,35(3):63-66,4.基金项目
新疆少数民族科技人才特殊培养计划科研项目(201423130) （201423130）
