中国动物检疫2018，Vol.35Issue(3)：67-71,5.DOI:10.3969/j.issn.1005-944X.2018.03.019
信号肽对溶血素在大肠杆菌中跨膜转运的影响
The Influence of Signal Peptide on Transmembrane Transportation of Suilysin in E. coli.
摘要
Abstract
Signal peptide is of great significance in the process of transmembrane transportation of foreign proteins. Suilysin genes with and without signal peptide sequence were obtained by PCR test,then the expression vectors were constructed and the fusion protein was induced and expressed in E.coli.By hemolytic test and Western-blot,it was confirmed that the recombinant suilysin without signal peptide could not be secreted by transmembrane transportation, while the recombinant suilysin with signal peptide could be secreted. This experiment was a useful exploration for researching gene attenuated vaccine of Streptococcus suis type 2,bacterial signal peptide and soluble expression of recombinant protein.关键词
溶血素/信号肽/大肠杆菌/跨膜转运Key words
suilysin/signal peptide/E.coli/transmembrane transportation分类
农业科技引用本文复制引用
齐安生,张秀娟,宫枫举,张兴进,马喆,潘子豪,孙学强..信号肽对溶血素在大肠杆菌中跨膜转运的影响[J].中国动物检疫,2018,35(3):67-71,5.基金项目
国家重点研发计划项目(2017YFC120050) （2017YFC120050）
