中国动物检疫2018，Vol.35Issue(3)：72-75,80,5.DOI:10.3969/j.issn.1005-944X.2018.03.019
15N稳定同位素标记副溶血弧菌的培养基制备与培育
Preparation of Culture Medium and cultivation of Vibrio Parahaemolyticus Labelled with 15N Stable Isotope
摘要
Abstract
In order to conduct quantitative study of proteomics and to develop Vibrio parahaemolyticus culture medium labelled with 15N stable isotope,by the method of 15N marking Vibrio parahaemolyticus,including repeatedly transferring and cultivating Vibrio parahaemolyticus and verifing the labeling efficiency of nitrogen-atoms in the Vibrio parahaemolyticus bacterial cells by LC-MS/MS,the culture medium was obtained.The formula of the culture medium was:NH4Cl(2 g/L),glucose(10 g/L),KH2PO4(0.6 g/L),KH2PO4(0.9 g/L),MgSO4(0.2 g/L),NaCl(20 g/L)and distilled water(1 L,pH 7.0-7.5). The nitrogenous sourced nitrogen-atoms were labeled by 15N,and the the labeling efficiency was 91.5%. The results showed that the technology was simple and reasonable,and its components were simple and low cost.As a conclusion,it would provide a new method for preparing Vibrio parahaemolyticus culture medium labeled with 15N in industry.关键词
15N/稳定同位素/副溶血弧菌/液相色谱-串联质谱Key words
15N/stable isotope/Vibrio parahaemolyticus/liquid chromatography-tandem mass spectrometry分类
农业科技引用本文复制引用
赵丽青,李正义,王勇,黄小华,张晓良,刘恩德,胡炜,孙宇,吴振兴,贾俊涛..15N稳定同位素标记副溶血弧菌的培养基制备与培育[J].中国动物检疫,2018,35(3):72-75,80,5.基金项目
国家质检总局科技计划项目(2013IK175、2015IK203、2016IK198、2014IK024) （2013IK175、2015IK203、2016IK198、2014IK024）
