中国动物检疫2018，Vol.35Issue(3)：86-89,103,5.DOI:10.3969/j.issn.1005-944X.2018.03.023
两种固相萃取柱在乙酰氨基阿维菌素药动学前处理中的比较
Effect Comparison of Two SPE Cartridges in Pretreatment of Pharmacokinetics Experiments of Eprinomectin
摘要
Abstract
In order to evaluate the purification and extraction effects of different brands of SPE columns in pretreatment of pharmacokinetics experiments of Eprinomectin(EPR). Firstly,a certain amount of EPR standard solution was added to blank fetal bovine serum. Methanol was used to purify EPR in serum. Then the solution was extracted by Agilent's and Waters' C18SPE columns respectively,and was derivatized with N-methylimidazole and trifluoroacetic anhydride. The final products were determined by ultra performance liquid chromatography-fluorescence detector. At the same time,an equal amount of EPR standard solution was derivatized and determined by ultra performance liquid chromatography-fluorescence detector,and the result was taken as the control group. Besides,the absolute recovery rate was the criteria for evaluating the two brands of SPE columns. The results showed that,after being extracted by Agilent's and Waters'C18SPE columns respectively,the two EPR peaks were sharp symmetry without obvious impurity peaks,but there was a difference in absolute recovery rates,which were 94.50% and 85.79%,respectively. As a conclusion,the C18SPE column of Agilent company was more effective for EPR extraction.关键词
乙酰氨基阿维菌素/固相萃取/SPE柱/超高效液相色谱-荧光检测Key words
Eprinomectin/solid phase extraction/SPE column/ultra performance liquid chromatography-fluorescence detection分类
农业科技引用本文复制引用
张振东,刘希望,马宁,申栋帅,李剑勇..两种固相萃取柱在乙酰氨基阿维菌素药动学前处理中的比较[J].中国动物检疫,2018,35(3):86-89,103,5.基金项目
国家重点研发计划专项(2016YFD0501306) （2016YFD0501306）
